Beati L, Meskini M, Thiers B, Raoult D
Unité des Rickettsies, Faculté de Médecine, Université de la Mediterranee, Marseille, France.
Int J Syst Bacteriol. 1997 Apr;47(2):548-54. doi: 10.1099/00207713-47-2-548.
We formally propose the name Rickettsia aeschlimannii sp. nov. for a new spotted fever group (SFG) rickettsia, strain MC16T, isolated from Hyalomma marginatum marginatum ticks collected in Morocco. This organism shows a typical rickettsial morphology when analyzed by electron microscopy. After characterization by serotyping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western immunoblotting, PCR-restriction fragment length polymorphism (RFLP), pulsed-field gel electrophoresis, and 16S rDNA sequencing, this organism was found to be different from all of the recognized SFG rickettsiae. Identical PCR-RFLP profiles have, however, been found in H. marginatum marginatum from Portugal and H. marginatum rufipes from Zimbabwe, which suggests that the distribution of this rickettsia reaches from the Mediterranean to southern Africa.
我们正式提议将从摩洛哥采集的边缘璃眼蜱中分离出的一株新的斑点热群（SFG）立克次体——菌株MC16T命名为阿氏立克次体（Rickettsia aeschlimannii）新种。通过电子显微镜分析，该生物体呈现出典型的立克次体形态。经过血清分型、十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳、Western免疫印迹、PCR - 限制性片段长度多态性（RFLP）、脉冲场凝胶电泳和16S rDNA测序等鉴定后，发现该生物体与所有已确认的SFG立克次体均不同。然而，在来自葡萄牙的边缘璃眼蜱和来自津巴布韦的红棕璃眼蜱中发现了相同的PCR - RFLP图谱，这表明这种立克次体的分布范围从地中海延伸至非洲南部。
