Kelly P J, Beati L, Mason P R, Matthewman L A, Roux V, Raoult D
Department of Clinical Veterinary Studies, University of Zimbabwe, Harare.
Int J Syst Bacteriol. 1996 Apr;46(2):611-4. doi: 10.1099/00207713-46-2-611.
We propose the name Rickettsia africae sp. nov. (with type strain Z9-Hu) for a distinct species of spotted fever group (SFG) rickettsiae that is the etiological agent of African tick bite fever in humans. This rickettsia has a distinct natural cycle and can be phenotypically distinguished from the other SFG rickettsiae by microimmunofluorescence serotyping, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and by Western blotting (immunoblotting). Genotypic differences between R. africae and the other SFG rickettsiae can be demonstrated by PCR restriction fragment length polymorphism analysis, pulsed-field gel electrophoresis, and sequencing of the 16S rRNA gene.
我们提议将一种独特的斑点热群（SFG）立克次氏体命名为非洲立克次氏体新种（模式菌株为Z9-Hu），它是人类非洲蜱咬热的病原体。这种立克次氏体有独特的自然循环，并且通过微量免疫荧光血清分型、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳以及蛋白质印迹法（免疫印迹法），可在表型上与其他SFG立克次氏体相区分。通过聚合酶链反应-限制性片段长度多态性分析、脉冲场凝胶电泳以及16S核糖体RNA基因测序，可证明非洲立克次氏体与其他SFG立克次氏体之间的基因型差异。
