Grayhack J T, Wendel E F, Oliver L, Lee C
J Urol. 1979 Mar;121(3):295-9. doi: 10.1016/s0022-5347(17)56760-9.
In an attempt to identify an indicator(s) specifically associated with prostatic cancer prostatic fluid was collected by rectal massage from patients with prostatic cancer, prostatitis, benign prostatic hyperplasia and from those without recognized prostatic lesions in order to measure various immunoproteins. The proteins examined were IgG, IgA, IgM, complements C3 and C4, and transferrin. Prostatic fluid samples were subjected first to immunoelectrophoresis. Distinct differences in C3, C4 and transferrin concentrations were noted between patients with prostatic cancer and other patients. These proteins were stained heavily in the electrophoresis gels of fluid from cancer patients but were either missing or lightly stained in all other groups. These qualitative determinations were replaced subsequently by a quantitative measurement using the radial immunodiffusion technique. Results of the latter study confirmed the aforementioned observations and indicated that the levels of C3, C4 and transferrin in the prostatic fluid of cancer patients were elevated significantly when compared to all other patient groups. These observations indicate that the measurement of complements C3 and C4, and transferrin in the prostatic fluid may assist in the identification of patients with a high risk of prostatic cancer.
为了确定与前列腺癌特别相关的指标，通过直肠按摩从前列腺癌、前列腺炎、良性前列腺增生患者以及无明显前列腺病变的患者中采集前列腺液，以检测各种免疫蛋白。检测的蛋白质包括IgG、IgA、IgM、补体C3和C4以及转铁蛋白。前列腺液样本首先进行免疫电泳。前列腺癌患者与其他患者之间的C3、C4和转铁蛋白浓度存在明显差异。这些蛋白质在癌症患者的前列腺液电泳凝胶中染色较深，但在所有其他组中要么缺失要么染色较浅。这些定性测定随后被采用放射免疫扩散技术的定量测量所取代。后一项研究的结果证实了上述观察结果，并表明与所有其他患者组相比，癌症患者前列腺液中C3、C4和转铁蛋白的水平显著升高。这些观察结果表明，检测前列腺液中的补体C3、C4和转铁蛋白可能有助于识别前列腺癌高危患者。
