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与施氏假单胞菌基因组变种相关的16S rRNA基因序列分析及巴利阿里假单胞菌新种的提议

16S rRNA gene sequence analysis relative to genomovars of Pseudomonas stutzeri and proposal of Pseudomonas balearica sp. nov.

作者信息

Bennasar A, Rosselló-Mora R, Lalucat J, Moore E R

机构信息

Departamento de Biología Ambiental, Universitat de les Illes Baleares, Palma de Mallorca, Spain.

出版信息

Int J Syst Bacteriol. 1996 Jan;46(1):200-5. doi: 10.1099/00207713-46-1-200.

DOI:10.1099/00207713-46-1-200
PMID:8573496
Abstract

We compared the 16S rRNA gene sequences of 14 strains of Pseudomonas stutzeri, including type strain CCUG 11256 and strain ZoBell (= ATCC 14405), which represented the seven P. stutzeri genomovars (DNA-DNA similarity groups) that have been described. Our sequence analysis revealed clusters which were highly correlated with genomovar clusters derived from DNA-DNA hybridization data. In addition, we identified signature nucleotide positions for each genomovar. We found that the 16S rRNA gene sequences of genomovar 6 strains SP1402T (T = type strain) and LS401 were different enough from the sequence of the type strain of P. stutzeri that these organisms should be placed in a new species, Pseudomonas balearica. The type strain of P. balearica is strain SP1402 (= DSM 6083).

摘要

我们比较了14株斯氏假单胞菌(Pseudomonas stutzeri)的16S rRNA基因序列,其中包括模式菌株CCUG 11256和菌株佐贝尔(ZoBell,= ATCC 14405),它们代表了已描述的七个斯氏假单胞菌基因组群(DNA-DNA相似性组)。我们的序列分析揭示了与源自DNA-DNA杂交数据的基因组群聚类高度相关的聚类。此外,我们确定了每个基因组群的特征性核苷酸位置。我们发现基因组群6菌株SP1402T(T = 模式菌株)和LS401的16S rRNA基因序列与斯氏假单胞菌模式菌株的序列差异足够大,以至于这些微生物应被归入一个新物种——巴利阿里假单胞菌(Pseudomonas balearica)。巴利阿里假单胞菌的模式菌株是菌株SP1402(= DSM 6083)。

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