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[用于检测不同类别免疫球蛋白的免疫比浊法检测的开发]

[Development of an IHI test for detection of immunoglobulins of different classes].

作者信息

Noskov F S, Konikova R E, Shakhanina K L, Malkina L A, Avdeenko M M

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1977 Feb(2):53-7.

PMID:857518
Abstract

The authors elaborated a method of obtaining stable erythrocytic diagnostic agent intended for titration of immunoglobulins A, M and G in the human blood serum by means of the indirect hemagglutination inhibition test (IHI). The suggested microdroplet method of conducting the IHI test permits to reveal (X +/- m): IgG--0.29 +/- 0.15 microng/ml, IgM--1.56 +/- 0.2 microng/ml, and IgA--0.16 +/- 0.07 microng/ml. The test is highly specific, simple and can be used in practical serological laboratories. The result is obtained in 2 1/2--3 hours.

摘要

作者阐述了一种获取稳定红细胞诊断剂的方法,该诊断剂用于通过间接血凝抑制试验(IHI)滴定人血清中的免疫球蛋白A、M和G。所建议的进行IHI试验的微滴法能够检测出(X±m):IgG为0.29±0.15微克/毫升,IgM为1.56±0.2微克/毫升,IgA为0.16±0.07微克/毫升。该试验具有高度特异性、操作简单,可用于实际血清学实验室。在2个半小时至3小时内可得出结果。

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