Washimine H, Asada Y, Kitamura K, Ichiki Y, Hara S, Yamamoto Y, Kangawa K, Sumiyoshi A, Eto T
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Histochem Cell Biol. 1995 Apr;103(4):251-4. doi: 10.1007/BF01457408.
The histological localization was investigated of adrenomedullin (AM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma. The immunohistological distribution was examined of AM in human, rat, and porcine tissues using a polyclonal antibody to a fragment comprising C-terminal amino acids 40-52 of human adrenomedullin [AM(40-52)NH2]. Almost all of the human pheochromocytoma and normal adrenal medullary cells of all three species were immunostained and found to be intensely positive for AM. Furthermore, AM-immunoreactive cells were present in the pancreatic islets, gastrointestinal neuroendocrine system, anterior pituitary, and choroid plexus with some degree of interspecies heterogeneity. These findings indicate that AM-immunoreactive cells are widely distributed in the endocrine and neuroendocrine system, suggesting that AM plays some important role in the control of systemic and local circulation and also of humoral secretion.
对肾上腺髓质素（AM）进行了组织学定位研究，肾上腺髓质素是一种最初从人嗜铬细胞瘤中分离出的新型血管舒张肽。使用针对人肾上腺髓质素C末端氨基酸40 - 52片段[AM(40 - 52)NH2]的多克隆抗体，检测了AM在人、大鼠和猪组织中的免疫组织化学分布。几乎所有三种物种的人嗜铬细胞瘤和正常肾上腺髓质细胞都被免疫染色，并且发现对AM呈强阳性。此外，胰岛、胃肠神经内分泌系统、垂体前叶和脉络丛中存在AM免疫反应性细胞，存在一定程度的种间异质性。这些发现表明，AM免疫反应性细胞广泛分布于内分泌和神经内分泌系统，提示AM在全身和局部循环以及体液分泌的控制中发挥一些重要作用。
