Curfs J H, Meis J F, Fransen J A, van der Lee H A, Hoogkamp-Korstanje J A
Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
Med Microbiol Immunol. 1995 Oct;184(3):123-7. doi: 10.1007/BF00224348.
The in vitro interactions of Yersinia enterocolitica, Salmonella typhimurium and Escherichia coli with polarized human colonic carcinoma (Caco-2) cells are described. Invasion of a confluent Caco-2 cell monolayer by Yersinia and Salmonella took place within 4 h after contact, which was in marked contrast to E. coli which did not invade Caco-2 cells. Cytoplasmic extrusions developed on the apical membrane and indicated the site of entrance of bacteria into the Caco-2 cells. Intracellular Yersinia and Salmonella were surrounded by a vacuolar membrane. Single as well as multiple bacteria were enclosed within a single vacuole. At 6 h after contact some of the intracellular yersiniae were found free in the cytoplasm. Furthermore, morphological signs of degeneration of Caco-2 cells such as vacuolization and autophagy were observed. Caco-2 cells infected with Salmonella also showed degenerative changes but the salmonellae resided within membrane-bound vacuoles in contrast to Yersinia. These observations are in contrast to those described for the invasion of other cells lines (not derived from intestinal epithelium) by Yersinia and may reflect more closely the interactions between Yersinia and the intestinal epithelium during gastrointestinal infection.
本文描述了小肠结肠炎耶尔森菌、鼠伤寒沙门菌和大肠杆菌与极化的人结肠癌细胞（Caco-2）在体外的相互作用。耶尔森菌和沙门菌在接触后4小时内即可侵袭汇合的Caco-2细胞单层，这与不侵袭Caco-2细胞的大肠杆菌形成鲜明对比。顶端膜上出现了细胞质突起，表明细菌进入Caco-2细胞的部位。细胞内的耶尔森菌和沙门菌被液泡膜包围。单个或多个细菌被包裹在单个液泡内。接触后6小时，一些细胞内的耶尔森菌在细胞质中游离。此外，还观察到Caco-2细胞退变的形态学迹象，如空泡化和自噬。感染沙门菌的Caco-2细胞也表现出退变变化，但与耶尔森菌不同的是，沙门菌存在于膜结合的液泡内。这些观察结果与耶尔森菌侵袭其他细胞系（非源自肠上皮）的描述不同，可能更密切地反映了胃肠道感染期间耶尔森菌与肠上皮之间的相互作用。
