Kishi M, Nakaya T, Nakamura Y, Kakinuma M, Takahashi T A, Sekiguchi S, Uchikawa M, Tadokoro K, Ikeda K, Ikuta K
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Med Microbiol Immunol. 1995 Oct;184(3):135-8. doi: 10.1007/BF00224350.
The presence of Borna disease virus (BDV) in peripheral blood mononuclear cells (PBMC) of 100 blood donors from Sapporo and 72 blood donors from Tokyo was examined using nested reverse transcriptase/polymerase chain reaction amplification with specific-primers for BDV p24. Anti-BDV p24 antibodies in the plasma of the 100 blood donors from Sapporo also were studied by enzyme-linked immunosorbent assay and by Western blot. BDV RNA was detected in 3 (4.2%) of the 72 PBMC samples from Tokyo, and in 5 (5%) of the 100 PBMC samples from Sapporo. In contrast, anti-p24 antibodies were found in only 1 (1%) of the donors from Sapporo. These results suggest that BDV infection in humans may be more widespread than previously thought.
使用针对博尔纳病病毒（BDV）p24的特异性引物进行巢式逆转录/聚合酶链反应扩增，检测了来自札幌的100名献血者和来自东京的72名献血者外周血单核细胞（PBMC）中BDV的存在情况。还通过酶联免疫吸附测定和蛋白质印迹法研究了来自札幌的100名献血者血浆中的抗BDV p24抗体。在来自东京的72份PBMC样本中，有3份（4.2%）检测到BDV RNA，在来自札幌的100份PBMC样本中，有5份（5%）检测到BDV RNA。相比之下，在来自札幌的献血者中，仅1名（1%）检测到抗p24抗体。这些结果表明，人类BDV感染可能比之前认为的更为普遍。
