George W L, Sutter V L, Goldstein E J, Ludwig S L, Finegold S M
Lancet. 1978 Apr 15;1(8068):802-3. doi: 10.1016/s0140-6736(78)93001-5.
Clostridium difficile was isolated from the faeces of a patient with clindamycin-associated pseudomembranous colitis (P.M.C.). The presence of a preformed faecal toxin and the toxigenicity of both the faecal isolate of C. difficile and a reference strain of C. difficile were demonstrated by tissue-culture assay. The toxin of both strains of C. difficile and that in the patient's faeces were neutralised by heating and by incubation with antitoxin to C. sordellii, but not by incubation with antitoxin to C. histolyticum, C. oedematiens (novyi), C. welchii (C. perfringens) or C. septicum. These data implicate the toxin of C. difficile as a major, and perhaps the sole, cause of antimicrobial-agent-associated P.M.C. of man and suggest that the neutralisation of the faecal toxin of P.M.C. by C. sordellii antitoxin, as described by other investigators, may be a non-specific phenomenon.
艰难梭菌是从一名患有克林霉素相关性假膜性结肠炎（P.M.C.）患者的粪便中分离出来的。通过组织培养试验证实了粪便中存在预先形成的毒素以及艰难梭菌粪便分离株和艰难梭菌参考菌株的产毒性。两种艰难梭菌菌株的毒素以及患者粪便中的毒素可通过加热以及与索氏梭菌抗毒素孵育而被中和，但与溶组织梭菌、水肿梭菌（诺维氏梭菌）、魏氏梭菌（产气荚膜梭菌）或败血梭菌抗毒素孵育则不能被中和。这些数据表明艰难梭菌毒素是人类抗菌药物相关性P.M.C.的主要原因，甚至可能是唯一原因，并提示其他研究者所描述的索氏梭菌抗毒素对P.M.C.粪便毒素的中和作用可能是一种非特异性现象。
