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来自酿酒酵母的七号染色体右臂上一个27千碱基片段的序列揭示了MOL1、NAT2、RPL30B、RSR1、CYS4、PEM1/CHO2、NSR1基因以及十个新的开放阅读框。

The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames.

作者信息

Skala J, Nawrocki A, Goffeau A

机构信息

Institute of Microbiology, Wroclaw University, Poland.

出版信息

Yeast. 1995 Nov;11(14):1421-7. doi: 10.1002/yea.320111410.

Abstract

The DNA sequence of a 26,677 bp fragment from the right arm of chromosome VII from Saccharomyces cerevisiae reveals 18 open reading frames (ORFs) longer than 300 bp. Eight ORFs correspond to previously characterized genes. G6620 is the 3' end of the MOL1 gene coding for a polypeptide similar to stress-inducible proteins from Fusarium; G6630 is the NAT2 gene which encodes a methionine N-acetyltransferase; G6635 is the RPL30B gene coding for the ribosomal protein L30; G6658 is RSR1 encoding a ras-related protein; G6667 is CYS4, the gene for cystathionine beta-synthase; G6670 is identical to ORF2 located close to CYS4; G6673 is PEM1/CHO2 encoding a phosphatidylethanolamine methyltransferase; G7001 is the NSR1 gene coding for a nuclear signal recognition protein. G6664 shares significant homology with the ORF YKR076w from chromosome XI. The other nine ORFs show no significant homology to any protein sequence presently available in the public data bases.

摘要

来自酿酒酵母七号染色体右臂的一个26,677 bp片段的DNA序列显示有18个大于300 bp的开放阅读框(ORF)。其中8个ORF与先前已鉴定的基因相对应。G6620是MOL1基因的3'末端,该基因编码一种与镰刀菌属的应激诱导蛋白相似的多肽;G6630是NAT2基因,它编码甲硫氨酸N - 乙酰转移酶;G6635是编码核糖体蛋白L30的RPL30B基因;G6658是编码ras相关蛋白的RSR1;G6667是胱硫醚β-合酶基因CYS4;G6670与位于CYS4附近的ORF2相同;G6673是编码磷脂酰乙醇胺甲基转移酶的PEM1/CHO2;G7001是编码核信号识别蛋白的NSR1基因。G6664与来自十一号染色体的ORF YKR076w有显著同源性。其他9个ORF与公共数据库中目前可获得的任何蛋白质序列均无显著同源性。

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