Bécam A M, Cullin C, Grzybowska E, Lacroute F, Nasr F, Ozier-Kalogeropoulos O, Palucha A, Slonimski P P, Zagulski M, Herbert C J
Centre de Génétique Moléculaire, Laboratoire propre du CNRS associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Yeast. 1994 Apr;10 Suppl A:S1-11. doi: 10.1002/yea.320100002.
We have determined the complete nucleotide sequence of a 29.7 kb segment from the right arm of chromosome II carried by the cosmid alpha 61. The sequence encodes the 3' region of the IRA1 gene and 13 complete open reading frames, of which ten correspond to new genes and three (CIF1, ATPsv and CKS1) have been sequenced previously. The density of protein coding sequences is particularly high and corresponds to 84% of the total length. Two new genes encode membrane proteins, one of which is particularly large, 273 kDa. In one case (ATPsv), the comparison of our sequence and the published sequence reveals significant differences.
我们已经确定了黏粒α61所携带的来自第二条染色体右臂的一段29.7 kb片段的完整核苷酸序列。该序列编码IRA1基因的3'区域和13个完整的开放阅读框，其中10个对应新基因，3个（CIF1、ATPsv和CKS1）先前已测序。蛋白质编码序列的密度特别高，占总长度的84%。两个新基因编码膜蛋白，其中一个特别大，为273 kDa。在一个案例（ATPsv）中，我们的序列与已发表序列的比较显示出显著差异。
