Hök K
Department of Medical Microbial Ecology, Karolinska Institute, Stockholm, Sweden.
Acta Vet Scand. 1991;32(2):171-6. doi: 10.1186/BF03546977.
An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.
采用瞬膜涂片的间接免疫荧光法（M3试验）诊断猫冠状病毒（FCV）感染，并与免疫组织病理学（对疑似猫传染性腹膜炎（FIP）的猫的器官进行间接免疫荧光测定（IFFA））和常规病理学（RP）进行比较。观察到两种免疫荧光方法（器官免疫荧光法（IFO）和M3）之间存在密切相关性。尽管M3试验仅需从每只动物的一个器官采集样本，但与IFO（平均从每只动物的5个器官采集样本）相比，其敏感性和特异性均较高（80%）。在21%疑似FIP的猫中发现了典型的病理病变。由于M3试验相对容易操作，它可以减轻病理实验室的工作量，并为临床和流行病学应用提供有价值的数据。
