Hök K
Department of Medical Microbial Ecology, Karolinska Institutet, Stockholm, Sweden.
APMIS. 1989 Sep;97(9):820-4. doi: 10.1111/j.1699-0463.1989.tb00483.x.
A simple technique demonstrating the presence of feline infectious peritonitis virus (FIPV) in cats is described. Smears from the third eyelid (membrana nictitans) were dried on microscope slides, fixed in aetone, and stained using an indirect immunofluorescence method with rabbit-anti-FIPV serum. By this method FIPV-antigen could be demonstrated in the cytoplasma of epithelial cells. The results obtained with this method (M3) were compared with serological and immunohistopathological methods for confirming FIPV-infection in cats. The results obtained by the M3 test were up to 85% in concordance with these other tests. Furthermore, the M3 test was easier and quicker to perform and should be well suited for clinical practice.
本文描述了一种用于检测猫体内猫传染性腹膜炎病毒（FIPV）的简单技术。取第三眼睑（瞬膜）涂片，在显微镜载玻片上干燥，用丙酮固定，然后用兔抗FIPV血清通过间接免疫荧光法染色。通过这种方法，可在上皮细胞的细胞质中检测到FIPV抗原。将该方法（M3）得到的结果与用于确诊猫FIPV感染的血清学和免疫组织病理学方法进行比较。M3检测得到的结果与其他检测结果的一致性高达85%。此外，M3检测操作更简便、快速，非常适合临床应用。
