White D J, Cox E R, Gwynn A V
The Procter and Gamble Company, Sharon Woods Technical Center, Cincinnati, Ohio, USA.
J Clin Dent. 1995;6 Spec No:84-8.
A Plaque Glycolysis and Regrowth Method (PGRM) has been used to examine the effects of single brushing with a new stabilized stannous fluoride dentifrice on plaque acid production. Plaque samples collected 45 minutes after subject toothbrushing were compared with samples collected prior to toothbrushing for glycolytic activity (pH reduction in incubation media), and the composition and proportion of acids produced during metabolism (capillary electrophoresis). Subjects brushed with both active SnF2 and placebo dentifrices in a cross-over design. Acetic and lactic acid were found to be produced during PGRM metabolism. SnF2 dentifrice was observed to significantly inhibit plaque glycolysis, reducing the pH drop associated with acid production. and specifically inhibiting both lactic acid and acetic acid production. Control dentifrice had a modest inhibitory effect on acid production, primarily inhibiting acetic acid production. SnF2 dentifrice inhibited lactic acid production more effectively than control dentifrice, and this effect appeared to be related to SnF2 specificity in metabolic inhibition, as opposed to the components of control dentifrice, namely ionic fluoride and surfactant. These experimental results support the in vivo antimicrobial activity of SnF2 formulated in a new stabilized matrix (currently marketed as Crest Gum Care), and demonstrate the utility of the PGRM method in elucidating specific mechanisms of action for antimicrobial agents.
采用菌斑糖酵解与再生长法（PGRM）来检测使用一种新型稳定化氟化亚锡牙膏单次刷牙对菌斑酸产生的影响。将受试者刷牙45分钟后采集的菌斑样本与刷牙前采集的样本进行比较，以检测糖酵解活性（培养基中pH值降低情况）以及代谢过程中产生的酸的组成和比例（毛细管电泳法）。受试者采用交叉设计分别使用含活性SnF2的牙膏和安慰剂牙膏刷牙。在PGRM代谢过程中发现产生了乙酸和乳酸。观察到含SnF2的牙膏能显著抑制菌斑糖酵解，减少与酸产生相关的pH值下降，并且特别抑制乳酸和乙酸的产生。对照牙膏对酸产生有适度的抑制作用，主要是抑制乙酸的产生。含SnF2的牙膏比对照牙膏更有效地抑制乳酸产生，而且这种作用似乎与SnF2在代谢抑制方面的特异性有关，而非对照牙膏的成分，即离子氟化物和表面活性剂。这些实验结果支持了以新型稳定化基质配方的SnF2（目前作为佳洁士牙龈护理牙膏上市）的体内抗菌活性，并证明了PGRM方法在阐明抗菌剂具体作用机制方面的实用性。
