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西方许旺酵母转化酶编码基因的克隆与序列分析

Cloning and sequence analysis of the gene encoding invertase from the yeast Schwanniomyces occidentalis.

作者信息

Klein R D, Poorman R A, Favreau M A, Shea M H, Hatzenbuhler N T, Nulf S C

机构信息

Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.

出版信息

Curr Genet. 1989 Sep;16(3):145-52. doi: 10.1007/BF00391470.

Abstract

The gene encoding invertase (INV) has been cloned from Schwanniomyces occidentalis. The enzyme consists of 533 amino acids, 8 potential glycosylation sites and has a 45% identity with the invertase from Saccharomyces cerevisiae. The proenzyme has a 22 amino acid signal sequence that has a high alpha-helical transmembrane potential which differs significantly from that predicted for the Saccharomyces cerevisiae enzyme.

摘要

已从西方施万酵母中克隆出编码转化酶(INV)的基因。该酶由533个氨基酸组成,有8个潜在的糖基化位点,与酿酒酵母的转化酶有45%的同源性。该酶原具有一个22个氨基酸的信号序列,具有高α-螺旋跨膜电位,这与酿酒酵母酶的预测值有显著差异。

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