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西方许旺酵母转化酶编码基因的克隆与序列分析

Cloning and sequence analysis of the gene encoding invertase from the yeast Schwanniomyces occidentalis.

作者信息

Klein R D, Poorman R A, Favreau M A, Shea M H, Hatzenbuhler N T, Nulf S C

机构信息

Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.

出版信息

Curr Genet. 1989 Sep;16(3):145-52. doi: 10.1007/BF00391470.

DOI:10.1007/BF00391470
PMID:2688929
Abstract

The gene encoding invertase (INV) has been cloned from Schwanniomyces occidentalis. The enzyme consists of 533 amino acids, 8 potential glycosylation sites and has a 45% identity with the invertase from Saccharomyces cerevisiae. The proenzyme has a 22 amino acid signal sequence that has a high alpha-helical transmembrane potential which differs significantly from that predicted for the Saccharomyces cerevisiae enzyme.

摘要

已从西方施万酵母中克隆出编码转化酶(INV)的基因。该酶由533个氨基酸组成,有8个潜在的糖基化位点,与酿酒酵母的转化酶有45%的同源性。该酶原具有一个22个氨基酸的信号序列,具有高α-螺旋跨膜电位,这与酿酒酵母酶的预测值有显著差异。

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本文引用的文献

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Schwanniomyces: a potential superyeast?施万酵母:一种潜在的超级酵母?
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Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.两个具有不同5'端且差异调控的mRNA编码酵母转化酶的细胞内形式和分泌形式。
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Codon selection in yeast.酵母中的密码子选择
Cloning and sequence analysis of the invertase gene INV 1 from the yeast Pichia anomala.
异常毕赤酵母蔗糖转化酶基因INV 1的克隆与序列分析
Curr Genet. 1996 Feb;29(3):234-40. doi: 10.1007/BF02221553.
4
Molecular structure of the SWA2 gene encoding an AMY1-related alpha-amylase from Schwanniomyces occidentalis.编码来自西方许旺酵母的一种与AMY1相关的α-淀粉酶的SWA2基因的分子结构。
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Purification and characterisation of an Aspergillus niger invertase and its DNA sequence.黑曲霉转化酶的纯化、特性鉴定及其DNA序列
Curr Genet. 1993 Jul-Aug;24(1-2):60-6. doi: 10.1007/BF00324666.
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Cloning, sequencing, and disruption of a levanase gene of Bacillus polymyxa CF43.多粘芽孢杆菌CF43果聚糖酶基因的克隆、测序及破坏
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Curr Genet. 1991 Aug;20(3):219-24. doi: 10.1007/BF00326236.
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A classification of glycosyl hydrolases based on amino acid sequence similarities.基于氨基酸序列相似性的糖基水解酶分类
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Patterns of amino acids near signal-sequence cleavage sites.信号序列切割位点附近的氨基酸模式。
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5
SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase.酿酒酵母的SUC1基因:蔗糖酶大(糖蛋白)、小(无糖)形式的结构基因。
Mol Cell Biol. 1981 May;1(5):469-74. doi: 10.1128/mcb.1.5.469-474.1981.
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Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
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Compilation of published signal sequences.已发表信号序列的汇编。
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64. doi: 10.1093/nar/12.13.5145.
8
The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence.酿酒酵母中分泌型转化酶是由编码信号序列的mRNA合成的。
Mol Cell Biol. 1983 Mar;3(3):439-47. doi: 10.1128/mcb.3.3.439-447.1983.
9
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