Qiu K, Tomita Y, Hashimoto M, Ohsawa M, Kawano K, Wu D M, Aozasa K
Department of Pathology, Suzhou 2nd People's Hospital, China.
Int J Cancer. 1997 Apr 10;71(2):155-8. doi: 10.1002/(sici)1097-0215(19970410)71:2<155::aid-ijc5>3.0.co;2-#.
Information on geographic differences of Epstein-Barr virus (EBV) positivity and association with HLA in gastric carcinoma are limited. Therefore, the association of gastric carcinoma with EBV was examined in 118 patients from Suzhou, China, where the incidence of nasopharyngeal carcinoma (NPC) is high, and in 216 patients from Osaka, Japan, where the incidence of NPC is low, NPC being one of the EBV-associated carcinomas. Expression of HLA-A2 was also examined in some of these cases. The EBV genome was evidenced by PCR and in the tumor cells by in situ hybridization in 7/90 and 9/151 of cases from Suzhou and Osaka, respectively. Immunohistochemistry revealed that cancer cells in all cases with EBV did not express latent membrane protein-I. Type A was found in all cases positive for EBV. Among several histologic and clinical factors, only age of patients showed a correlation with EBV positivity: patients over 60 showed a higher frequency than patients below 60 years of age (p < 0.05). Typing of the HLA-A locus was possible in 16 cases positive for the EBV genome: 3 of 4 cases from Suzhou and 4 of 7 from Osaka were positive for HLA-A2 products. Severe lymphoid cell infiltration was found in 2 of 7 cases and 1 of 4 cases with and without the HLA-A2 type, respectively. The reported frequency of EBV positivity in Chinese living in Taiwan and in Japanese living in Hawaii is higher than in Suzhou, China, and Osaka, Japan, respectively. Our findings suggest that EBV association with gastric carcinoma is influenced by environmental and cultural factors.
关于爱泼斯坦-巴尔病毒（EBV）阳性在地理上的差异以及与胃癌中HLA关联的信息有限。因此，我们对来自中国苏州的118例患者和来自日本大阪的216例患者的胃癌与EBV的关联进行了研究。苏州地区鼻咽癌（NPC）发病率高，大阪地区NPC发病率低，而NPC是与EBV相关的癌症之一。我们还对其中一些病例检测了HLA - A2的表达。通过PCR证实了EBV基因组的存在，并且通过原位杂交在苏州和大阪的病例中分别在7/90和9/151的肿瘤细胞中检测到EBV基因组。免疫组织化学显示，所有EBV阳性病例的癌细胞均不表达潜伏膜蛋白-I。在所有EBV阳性病例中均发现了A型。在几个组织学和临床因素中，只有患者年龄与EBV阳性相关：60岁以上患者的EBV阳性频率高于60岁以下患者（p < 0.05）。对16例EBV基因组阳性病例进行了HLA - A位点分型：苏州的4例中有3例，大阪的7例中有4例HLA - A2产物呈阳性。在有和没有HLA - A2型的7例和4例病例中，分别有2例和1例发现严重的淋巴细胞浸润。据报道，居住在台湾的中国人和居住在夏威夷的日本人中EBV阳性频率分别高于中国苏州和日本大阪。我们的研究结果表明，EBV与胃癌的关联受环境和文化因素影响。
