Zhang M, Song G, Minuk G Y
Department of Digestive Medicine, First Teaching Hospital, Norman Bethune University of Medical Sciences, ChangChun, Jilin, China.
Gastroenterology. 1996 Apr;110(4):1150-5. doi: 10.1053/gast.1996.v110.pm8613004.
BACKGROUND & AIMS: Cirrhosis is a potentially lethal condition for which there is no proven effective therapy. The aim of this study was to compare the effects of hepatic stimulator substance, traditional Chinese herbal medicine, selenium plus vitamin E, and ciprofloxacin treatment on biochemical and histological features of fibrosis in rats with carbon tetrachloride (CCl4)/ethanol-induced cirrhosis.
One hundred twenty adult Wistar rats were divided into six study groups (20 rats/group): healthy controls, CCl4/ethanol-injured rats left untreated, and CCl4/ethanol-injured rats treated for 4 weeks with either hepatic stimulator substance, traditional Chinese herbal medicine, a combination of selenium plus vitamin E, or ciprofloxacin. After the 4-week treatment, rats were killed and the following parameters of hepatic fibrosis were determined: hepatic hydroxyproline and proline levels, serum hyaluronic acid concentrations, and histological staining of hepatic tissue.
Hepatic fibrosis was significantly improved in all four treated groups compared with the untreated CCl4/ethanol-injured controls. Improvements were most striking in the groups treated with traditional Chinese herbal medicine and ciprofloxacin.
The data indicate that hepatic stimulator substance, traditional Chinese herbal medicine, selenium plus vitamin E, and ciprofloxacin significantly decrease the amount of hepatic fibrosis caused by CCl4/ethanol injury in rats.
肝硬化是一种潜在的致命疾病，目前尚无经证实的有效治疗方法。本研究旨在比较肝刺激物质、中药、硒加维生素E以及环丙沙星治疗对四氯化碳（CCl4）/乙醇诱导的肝硬化大鼠纤维化的生化和组织学特征的影响。
将120只成年Wistar大鼠分为六个研究组（每组20只）：健康对照组、未治疗的CCl4/乙醇损伤大鼠组，以及用肝刺激物质、中药、硒加维生素E组合或环丙沙星治疗4周的CCl4/乙醇损伤大鼠组。4周治疗后，处死大鼠并测定以下肝纤维化参数：肝羟脯氨酸和脯氨酸水平、血清透明质酸浓度以及肝组织的组织学染色。
与未治疗的CCl4/乙醇损伤对照组相比，所有四个治疗组的肝纤维化均有显著改善。中药和环丙沙星治疗组的改善最为显著。
数据表明，肝刺激物质、中药、硒加维生素E以及环丙沙星可显著降低大鼠CCl4/乙醇损伤所致的肝纤维化程度。
