Roeder B L, Schaalje B, Kelly E J, Clark F D
Department of Animal Science, College of Biology and Agriculture, Brigham Young University, Provo, UT 84602, USA.
J Am Vet Med Assoc. 1996 Mar 1;208(5):707-10.
To determine an accurate rapid method for determination of blood glucose concentration in cattle under field conditions.
Prospective, randomized, controlled trial.
62 clinically normal Holstein cattle: 34 cows and 28 calves.
Glucose concentrations in venous blood samples were measured in duplicate using a rapid, dry-slide chemistry technique for determination of blood glucose concentration and a laboratory-based method for determination of plasma glucose concentration. Analyses of variance were used to determine whether the relationship between results of the 2 methods was affected by the status of the animals (cows vs calves) or the PCV of the blood samples. Simple linear regression was performed to determine the correlation between the 2 methods and the slope, intercept, and residual error variance of the relationship between the methods.
There as a significant linear relationship between the 2 methods throughout the range of glucose concentrations. Mean difference between results of the 2 methods (results for laboratory-based method - results for rapid method) was 12.95 mg/dl (SD, 7.20 mg/dl). The PCV did not affect the relationship, and there was no difference between results of the 2 methods for cows versus calves. Correlation between means of the duplicative values determined by use of the 2 methods was high (r = 0.9462).
The good correlation between the 2 procedures and the comparable precision estimates (coefficient of variation, 7.17% for laboratory-based method; coefficient of variation, 10.11% for rapid methods) indicates that using the rapid method to measure blood glucose concentration is valid in cows and calves.
确定一种在野外条件下测定牛血糖浓度的准确快速方法。
前瞻性、随机、对照试验。
62头临床正常的荷斯坦牛，其中34头母牛和28头犊牛。
静脉血样中的葡萄糖浓度采用快速干片化学技术测定血糖浓度进行双份测定，并采用基于实验室的方法测定血浆葡萄糖浓度。采用方差分析来确定两种方法的结果之间的关系是否受动物状态（母牛与犊牛）或血样的红细胞压积影响。进行简单线性回归以确定两种方法之间的相关性以及方法之间关系的斜率、截距和残余误差方差。
在整个葡萄糖浓度范围内，两种方法之间存在显著的线性关系。两种方法结果的平均差异（基于实验室方法的结果 - 快速方法的结果）为12.95mg/dl（标准差，7.20mg/dl）。红细胞压积不影响这种关系，母牛和犊牛的两种方法结果之间没有差异。使用两种方法测定的双份值均值之间的相关性很高（r = 0.9462）。
两种方法之间良好的相关性以及可比的精密度估计值（基于实验室方法的变异系数为7.17%；快速方法的变异系数为10.11%）表明，使用快速方法测量母牛和犊牛的血糖浓度是有效的。
