Khan Z M, Sebenik M, Zucker-Franklin D
Department of Medicine, New York University Medical Center, New York, USA.
J Invest Dermatol. 1996 Apr;106(4):667-72. doi: 10.1111/1523-1747.ep12345488.
The histopathologic diagnosis of mycosis fungoides (MF), even when clinical manifestations of the disease seem convincing, is often tenuous. The observation that practically all patients with MF harbor human T cell lymphotropic virus type I (HTLV-I) proviral sequences in their circulating lymphocytes raised the possibility that such viral footprints could be detected in their cutaneous infiltrates. Application of in situ polymerase chain reaction (PCR) to skin biopsies of 11 of 12 patients demonstrated this assumption to be correct. In addition, cells suspected to be keratinocytes were also positive. None of 10 skin biopsies from a variety of sources used as controls, nor 3 lymph node biopsies from patients with B-cell lymphomas, showed any HTLV proviral sequences on in situ PCR. On the basis of these observations, it is concluded that in situ PCR carried out on skin biopsies of patients with presumptive MF may help to established the diagnosis.
蕈样肉芽肿（MF）的组织病理学诊断，即便疾病的临床表现看似确凿，往往也不明确。几乎所有MF患者的循环淋巴细胞中都携带I型人类T细胞嗜淋巴细胞病毒（HTLV-I）前病毒序列，这一发现增加了在其皮肤浸润中检测到此类病毒痕迹的可能性。对12例患者中的11例进行皮肤活检并应用原位聚合酶链反应（PCR），结果表明这一假设是正确的。此外，疑似角质形成细胞的细胞也呈阳性。用作对照的10份来自各种来源的皮肤活检样本，以及3份B细胞淋巴瘤患者的淋巴结活检样本，在原位PCR中均未显示任何HTLV前病毒序列。基于这些观察结果，得出结论：对疑似MF患者进行皮肤活检并进行原位PCR可能有助于确诊。
