Shaheen A A, Abd El-Fattah A, Gad M Z
Department of Biochemistry, Faculty of Pharmacy, Cairo University, Egypt.
Experientia. 1996 Apr 15;52(4):336-9. doi: 10.1007/BF01919536.
The level of malondialdehyde (MDA), an index of lipid peroxidation, and the antioxidants superoxide dismutase (SOD) and glutathione (GSH), as well as the activity of Na+, K(+)-ATPase, were assessed in whole rat brain after immobilization, anemic hypoxia (NaNO2) and 72 h starvation. The effect of these stressors on plasma glucose and corticosterone levels was also observed. Hypoxia and starvation stimulated the lipid peroxide formation in brain as indicated by an increase in the level of MDA, being higher after starvation than hypoxia. Brain SOD activity was also increased in response to hypoxia and starvation while GSH content was only diminished in hypoxia. However, neither MDA nor antioxidants were affected by immobilization. On the other hand, the activity of brain Na+, K(+)-ATPase was significantly increased by immobilization and hypoxia but decreased in starvation. A similar pattern of change was also observed in plasma glucose and corticosterone levels in response to these stressors. These results elucidate differences in the biochemical response of animals towards various types of stress, with increased lipid peroxide formation in hypoxia and starvation.
在大鼠经历固定、贫血性缺氧（亚硝酸钠）和72小时饥饿后，对其全脑丙二醛（MDA，脂质过氧化指标）、抗氧化剂超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的水平以及Na +，K（+）-ATPase的活性进行了评估。还观察了这些应激源对血浆葡萄糖和皮质酮水平的影响。缺氧和饥饿刺激了大脑中脂质过氧化物的形成，这表现为MDA水平升高，饥饿后的MDA水平高于缺氧后的水平。大脑SOD活性也因缺氧和饥饿而增加，而GSH含量仅在缺氧时减少。然而，MDA和抗氧化剂均不受固定的影响。另一方面，大脑Na +，K（+）-ATPase的活性因固定和缺氧而显著增加，但在饥饿时降低。在这些应激源作用下，血浆葡萄糖和皮质酮水平也观察到类似的变化模式。这些结果阐明了动物对各种类型应激的生化反应差异，缺氧和饥饿时脂质过氧化物形成增加。
