Tanemura M, Suzumori K, Yagami Y, Katow S
Department of Obstetrics and Gynecology, Nagoya City University, Nagoya, Japan.
Am J Obstet Gynecol. 1996 Feb;174(2):578-82. doi: 10.1016/s0002-9378(96)70431-3.
Our purpose was to develop a reliable method for prenatal diagnosis of fetal rubella infection through the detection of viral ribonucleic acid extracted from the chorionic villi, amniotic fluid, or fetal blood in pregnant women.
Double amplification of rubella viral ribonucleic acid by nested polymerase chain reaction after reverse transcription was applied to samples from 34 women suspected of having rubella. The results were compared with those of serum antibody and levels of rubella virus-specific immunoglobulin M antibodies in fetal blood.
Viral ribonucleic acid was revealed in 8 of 34 cases (23.5%). In the remaining 26 cases, healthy babies were born in 24, 1 was electively aborted, and 1 died in the thirty-sixth week of pregnancy of unknown causes.
This method allowed very early detection of fetal rubella infection by sampling of chorionic villi and amniotic fluid compared with evaluation of the maternal symptoms and serum antibody levels. Fetal blood was also more useful for making a diagnosis up to the twentieth week of pregnancy than was measuring rubella virus-specific immunoglobulin M antibodies.
我们的目的是通过检测从孕妇的绒毛膜绒毛、羊水或胎儿血液中提取的病毒核糖核酸，开发一种可靠的胎儿风疹感染产前诊断方法。
对34名疑似风疹的女性样本应用逆转录后巢式聚合酶链反应对风疹病毒核糖核酸进行双重扩增。将结果与血清抗体以及胎儿血液中风疹病毒特异性免疫球蛋白M抗体水平的结果进行比较。
34例中有8例（23.5%）检测到病毒核糖核酸。其余26例中，24例出生健康婴儿，1例选择性流产，1例在妊娠36周时原因不明死亡。
与评估母亲症状和血清抗体水平相比，该方法通过绒毛膜绒毛和羊水采样能够非常早期地检测胎儿风疹感染。在妊娠20周之前，胎儿血液对于诊断也比检测风疹病毒特异性免疫球蛋白M抗体更有用。
