Walsh M C, Scholte M, Valkier J, Smits H P, van Dam K
E.C. Slater Institute, BioCentrum, University of Amsterdam, The Netherlands.
J Bacteriol. 1996 May;178(9):2593-7. doi: 10.1128/jb.178.9.2593-2597.1996.
The kinetics of glucose transport in a number of different mutants of Saccharomyces cerevisiae with multiple deletions in the glucose transporter gene family were determined. The deletions led to differences in maximal rate and affinity for glucose uptake by the cells, dependent on the growth conditions. At the same time, there were changes in glucose repression, as determined by expression of invertase activity. Only in the strain with genes HXT1-4 and SNF3 deleted but carrying HXT6/7 were glucose uptake kinetics and invertase activity independent of the presence or concentration of glucose in the growth medium. Some degree of glucose sensitivity was recovered if the SNF3 or HXT2 gene was present in the multiple-deletion background. It is hypothesized that during growth on glucose, both modulation of the kinetics of glucose uptake and derepression of invertase activity require the presence of more than one active gene of the glucose transporter family.
测定了酿酒酵母多个不同突变体中葡萄糖转运的动力学，这些突变体在葡萄糖转运蛋白基因家族中存在多处缺失。这些缺失导致细胞对葡萄糖摄取的最大速率和亲和力存在差异，这取决于生长条件。同时，通过转化酶活性的表达确定，葡萄糖阻遏存在变化。只有在缺失基因HXT1 - 4和SNF3但携带HXT6/7的菌株中，葡萄糖摄取动力学和转化酶活性才与生长培养基中葡萄糖的存在或浓度无关。如果在多重缺失背景中存在SNF3或HXT2基因，则会恢复一定程度的葡萄糖敏感性。据推测，在葡萄糖上生长期间，葡萄糖摄取动力学的调节和转化酶活性的去阻遏都需要葡萄糖转运蛋白家族中不止一个活性基因的存在。
