Neuveglise C, Sarfati J, Latge J P, Paris S
Laboratoire des Aspergillus, Institut Pasteur, Paris, France.
Nucleic Acids Res. 1996 Apr 15;24(8):1428-34. doi: 10.1093/nar/24.8.1428.
A repeated DNA sequence used for epidemiological studies of the human opportunistic pathogen Aspergillus fumigatus has been characterized. It is a retroelement of 6914 bp in length, bounded by long terminal repeats of 282 bp, with sequence and features characteristic of retroviruses and retrotransposons. A 5 bp duplication site was found at its borders. This element, designated Afut1, encodes amino acid sequences homologous to the reverse transcriptase, RNase H and endonuclease encoded by the pol genes of retroelements. Comparison of the peptidic sequences with other putative polypeptides of fungal LTR retrotransposons showed that Afut1 is a member of the gypsy group. This is the first report of a transposable element in A.fumigatus. Afut1 is a defective element: the putative coding domains contain multiple stop codons due exclusively to transitions from C:G to T:A.
一种用于人类机会致病菌烟曲霉流行病学研究的重复DNA序列已得到表征。它是一个长度为6914 bp的反转录元件，由282 bp的长末端重复序列界定，具有逆转录病毒和反转录转座子的序列及特征。在其边界发现了一个5 bp的重复位点。该元件命名为Afut1，编码与反转录元件pol基因所编码的逆转录酶、核糖核酸酶H和内切核酸酶同源的氨基酸序列。将肽序列与真菌LTR反转录转座子的其他推定多肽进行比较表明，Afut1是吉普赛族的成员。这是关于烟曲霉中可转座元件的首次报道。Afut1是一个缺陷元件：推定的编码结构域仅由于从C:G到T:A的转换而包含多个终止密码子。
