通过DNA扩增检测莱姆关节炎患者滑膜组织样本中的伯氏疏螺旋体。
Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with Lyme arthritis.
作者信息
Jaulhac B, Chary-Valckenaere I, Sibilia J, Javier R M, Piémont Y, Kuntz J L, Monteil H, Pourel J
机构信息
Institut de Bactériologie, Université Louis Pasteur, Strasbourg, France.
出版信息
Arthritis Rheum. 1996 May;39(5):736-45. doi: 10.1002/art.1780390505.
OBJECTIVE
To compare the detection rates of chromosomal flagellin gene from Borrelia burgdorferi in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques.
METHODS
B burgdorferi DNA was sought in SF and ST from 12 consecutive patients with Lyme arthritis and from 29 patients with noninfectious diseases (controls).
RESULTS
No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdorferi DNA was detected in all ST and/or SF samples from the 12 patients with Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the 11 remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05).
CONCLUSION
These data suggest that detection of chromosomal B burgdorferi DNA may be more efficient in ST than SF.
目的
运用聚合酶链反应（PCR）技术比较滑膜组织（ST）和滑液（SF）中伯氏疏螺旋体染色体鞭毛蛋白基因的检出率。
方法
对12例连续性莱姆关节炎患者以及29例非感染性疾病患者（对照组）的滑液和滑膜组织进行伯氏疏螺旋体DNA检测。
结果
29例对照患者的样本未观察到DNA扩增，而12例莱姆关节炎患者的所有滑膜组织和/或滑液样本均检测到伯氏疏螺旋体DNA。1例滑膜组织样本结果因PCR抑制剂而无法解读。在其余11例患者中，10例滑膜组织样本呈阳性，而滑液样本仅4例呈阳性（P < 0.05）。
结论
这些数据表明，滑膜组织中染色体伯氏疏螺旋体DNA的检测可能比滑液更有效。
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