转录因子Sp1和Oct-1通过物理相互作用来调节人类U2小核RNA基因的表达。
The transcription factors Sp1 and Oct-1 interact physically to regulate human U2 snRNA gene expression.
作者信息
Ström A C, Forsberg M, Lillhager P, Westin G
机构信息
Department of Medical Genetics, Uppsala University, Biomedical Centre, Uppsala, Sweden.
出版信息
Nucleic Acids Res. 1996 Jun 1;24(11):1981-6. doi: 10.1093/nar/24.11.1981.
Abstract
The expression of human small nuclear U2 RNA genes is controlled by the proximal sequence element (PSE), which determines the start site of transcription, and a distal sequence element (DSE). The DSE contains an octamer element and three Sp1 binding sites. The octamer, like the PSE, is essential for U2 transcription. The Sp1 sites contribute to full promoter activity by distance-dependent cooperative interactions with the transcription factors Sp1 and Oct-1. Here we show that purified recombinant Sp1 and Oct-1 bind cooperatively to the DSE and that they physically interact in vitro. Furthermore, we show that Sp1 and Oct-1 interact in vivo using a yeast two-hybrid system. The domain of Sp1 which interacts with Oct-1 is confined to the region necessary for transcriptional stimulation of U2 RNA transcription. This region contains the glutamine-rich activation domain B and a serine/threonine-rich part. The results demonstrate that Sp1, in addition to binding to a number of other factors, also interacts directly with transcription factor Oct-1.
摘要
人类小核U2 RNA基因的表达受近端序列元件（PSE）和远端序列元件（DSE）控制，PSE决定转录起始位点，DSE包含一个八聚体元件和三个Sp1结合位点。八聚体与PSE一样，对U2转录至关重要。Sp1位点通过与转录因子Sp1和Oct-1的距离依赖性协同相互作用促进启动子的完全活性。在此我们表明，纯化的重组Sp1和Oct-1协同结合至DSE，且它们在体外发生物理相互作用。此外，我们利用酵母双杂交系统表明Sp1和Oct-1在体内相互作用。与Oct-1相互作用的Sp1结构域局限于U2 RNA转录的转录刺激所需区域。该区域包含富含谷氨酰胺的激活结构域B和富含丝氨酸/苏氨酸的部分。结果表明，Sp1除了与许多其他因子结合外，还直接与转录因子Oct-1相互作用。
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