哺乳期大鼠培养肝细胞中极低密度脂蛋白分泌对胰岛素抑制作用的敏感性降低。
Decreased sensitivity of very-low-density lipoprotein secretion to the inhibitory effect of insulin in cultured hepatocytes from lactating rats.
作者信息
Bourgeois C S, Gibbons G F
机构信息
Metabolic Research Laboratory, University of Oxford, Radcliffe Infirmary, U.K.
出版信息
Biochem J. 1996 Jun 15;316 ( Pt 3)(Pt 3):737-41. doi: 10.1042/bj3160737.
Hepatocytes were prepared from 10-11-day lactating rat dams and from lactating dams which had been weaned for periods of either 1-2 days or 7 days. Hepatocytes from each group were cultured for periods of up to 48 h in a chemically defined medium. Compared with those from the 7-day weaned animals, hepatocytes from the lactating rats were resistant to the inhibitory effects of insulin on the secretion of very-low-density lipoprotein (VLDL) triacylglycerol (TAG). These differences persisted for up to 48 h in culture. Hepatocytes from the 1-2 day weaned animals remained relatively insulin-resistant in this respect. Similar differences in the response to insulin were not observed for the secretion of VLDL apolipoprotein B. TAG production increased and ketogenesis decreased in the hepatocytes from the lactating compared with those from the 7-day weaned rats. Insensitivity of the liver to the normal effects of insulin on the secretion of VLDL TAG may arise from a need to maintain an adequate flux of hepatic lipids to the lactating mammary gland in order to meet the large demand for milk-fat production.
肝细胞取自10 - 11日龄的哺乳期大鼠母鼠以及已断奶1 - 2天或7天的哺乳期母鼠。每组肝细胞在化学成分明确的培养基中培养长达48小时。与7日龄断奶动物的肝细胞相比，哺乳期大鼠的肝细胞对胰岛素抑制极低密度脂蛋白（VLDL）三酰甘油（TAG）分泌的作用具有抗性。这些差异在培养过程中持续长达48小时。在这方面，1 - 2日龄断奶动物的肝细胞仍相对具有胰岛素抗性。对于VLDL载脂蛋白B的分泌，未观察到对胰岛素反应的类似差异。与7日龄断奶大鼠的肝细胞相比，哺乳期肝细胞中TAG生成增加而酮生成减少。肝脏对胰岛素对VLDL TAG分泌的正常作用不敏感，可能是为了维持足够的肝脂质通量至哺乳期乳腺，以满足对乳脂肪产生的大量需求。
Zotero 插件