fts突变体中的FtsZ环形成。
FtsZ ring formation in fts mutants.
作者信息
Addinall S G, Bi E, Lutkenhaus J
机构信息
Department of Microbiology, Molecular Genetics and Immunology, Kansas 66160, USA.
出版信息
J Bacteriol. 1996 Jul;178(13):3877-84. doi: 10.1128/jb.178.13.3877-3884.1996.
Abstract
The formation of FtsZ rings (Z rings) in various fts mutants was examined by immunoelectron microscopy and immunofluorescence. In two temperature-sensitive ftsZ mutants which form filaments with smooth morphology, the Z ring was unable to form. In ftsA, ftsI, and ftsQ mutants, which form filaments with an indented morphology, Z rings formed but their contraction was blocked. These results indicate that fully functional ftsA, ftsQ, and ftsI genes are not required for Z-ring formation and are unlikely to have a role in localization of the Z ring. The results also suggest that one function of the Z ring is to localize the activity of other fts gene products.
摘要
通过免疫电子显微镜和免疫荧光检查了各种fts突变体中FtsZ环（Z环）的形成情况。在两个形成形态光滑的丝状菌体的温度敏感型ftsZ突变体中，Z环无法形成。在形成形态有凹陷的丝状菌体的ftsA、ftsI和ftsQ突变体中，Z环能够形成，但它们的收缩受到了阻碍。这些结果表明，Z环的形成并不需要功能完全正常的ftsA、ftsQ和ftsI基因，而且这些基因不太可能在Z环的定位中发挥作用。结果还表明，Z环的一个功能是定位其他fts基因产物的活性。
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