Heller R, Brown K E, Burgtorf C, Brown W R
Cancer Research Campaign Chromosome Molecular Biology Group, Biochemistry Department, Oxford University, England.
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7125-30. doi: 10.1073/pnas.93.14.7125.
We have used telomeric DNA to break two acrocentric derivatives of the human Y chromosome into mini-chromosomes that are small enough to be size- fractionated by pulsed-field gel electrophoresis. One of the mini-chromosomes is about 7 Mb in size and sequence-tagged site analysis of this molecule suggests that it corresponds to a simple truncation of the short arm of the Y chromosome. Five of the mini-chromosomes are derived from the long arm, are all rearranged by more than a simple truncation, and range in size from 4.0 Mb to 9 Mb. We have studied the mitotic stabilities of these mini-chromosomes and shown that they are stably maintained by cells proliferating in culture for about 100 cell divisions.
