Zimatkin S M, Karpuk Iu G
Morfologiia. 1994 Apr-Jun;106(4-6):83-91.
Immunocytochemical study of regional and cellular distribution of mitochondrial high-affinity aldehyde dehydrogenase (MA AlDH) in the rat CNS was performed by streptavidin-biotin-peroxidase method. Specific immunoreactivity of diverse intensity was found in most structures of brain and spinal cord regions mainly in neuronal and gliocyte cytoplasm and to a smaller extent, in their processes, terminations (neuropile conducting tracts, white matter) and blood vessels: immune reaction intensity of perikarya of different type neurons exceeds those of neuropile 1.8-3.3 times. MA AlDH positive neurons were observed in all the cerebral regions, with the most intensive specific staining in inferior olives and hippocamp pyramidal layer (0.500-0.520 optical density units) and the minimal in nucleus rubrum and substantia nigra (0.260-0.290 units). In both cases MA AlDH immunoreactive neurons made only part (from 40 to 88) of morphologically identified (thianine-positive) neurons. Comparative analysis of histochemical preparations and the cytophotometric results revealed significant diversities in topographic distribution of MA AlDG and general AlDG activity in the cerebrum. The data obtained assist in elucidation the spatial organization of alcohol and aldehyde metabolism in CNS and in comprehension the reasons of different sensitivity of brain structures to alcohol.
采用链霉亲和素-生物素-过氧化物酶法对大鼠中枢神经系统中线粒体高亲和力醛脱氢酶（MA AlDH）的区域和细胞分布进行免疫细胞化学研究。在脑和脊髓区域的大多数结构中发现了不同强度的特异性免疫反应，主要存在于神经元和神经胶质细胞的细胞质中，在其突起、终末（神经毡传导束、白质）和血管中的程度较小：不同类型神经元的胞体免疫反应强度比神经毡高1.8 - 3.3倍。在所有脑区均观察到MA AlDH阳性神经元，在下橄榄核和海马锥体细胞层特异性染色最强（光密度单位为0.500 - 0.520），在红核和黑质中最弱（0.260 - 0.290单位）。在这两种情况下，MA AlDH免疫反应性神经元仅占形态学鉴定（硫堇阳性）神经元的一部分（40%至88%）。对组织化学制剂和细胞光度测量结果的比较分析揭示了大脑中MA AlDG和总AlDG活性在地形分布上的显著差异。所获得的数据有助于阐明中枢神经系统中酒精和醛代谢的空间组织，并有助于理解脑结构对酒精不同敏感性的原因。
