50S核糖体亚基的合成与翻译是红霉素抑制金黄色葡萄球菌的等效靶点。
50S ribosomal subunit synthesis and translation are equivalent targets for erythromycin inhibition in Staphylococcus aureus.
作者信息
Champney W S, Burdine R
机构信息
Department of Biochemistry, J.H.Quillen College of Medicine, East Tennessee, State University, Johnson City 37614, USA.
出版信息
Antimicrob Agents Chemother. 1996 May;40(5):1301-3. doi: 10.1128/AAC.40.5.1301.
Abstract
Macrolide antibiotics like erythromycin can prevent the formation of the 50S ribosomal subunit in growing bacterial cells, in addition to their inhibitory effect on translation. The significance of this novel finding has been further investigated. The 50% inhibitory doses of erythromycin for the inhibition of translation and 50S subunit assembly in Staphylococcus aureus cells were measured and were found to be identical. Together they account quantitatively for the observed effects of erythromycin on cell growth rates. There is also a direct relationship between the loss of rRNA from the 50S subunit and its accumulation as oligoribonucleotides in cells. The importance of this second site for erythromycin inhibition of bacterial cell growth is discussed.
摘要
除了对翻译有抑制作用外，像红霉素这样的大环内酯类抗生素还能阻止正在生长的细菌细胞中50S核糖体亚基的形成。这一新发现的意义已得到进一步研究。测定了红霉素对金黄色葡萄球菌细胞翻译抑制和50S亚基组装的50%抑制剂量，发现二者相同。它们共同定量解释了观察到的红霉素对细胞生长速率的影响。50S亚基中rRNA的丢失与其在细胞中作为寡核糖核苷酸的积累之间也存在直接关系。本文讨论了这第二个作用位点对于红霉素抑制细菌细胞生长的重要性。
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本文引用的文献
1
Macrolide antibiotics inhibit 50S ribosomal subunit assembly in Bacillus subtilis and Staphylococcus aureus.大环内酯类抗生素抑制枯草芽孢杆菌和金黄色葡萄球菌中50S核糖体亚基的组装。
Antimicrob Agents Chemother. 1995 Sep;39(9):2141-4. doi: 10.1128/AAC.39.9.2141.
2
Erythromycin inhibits the assembly of the large ribosomal subunit in growing Escherichia coli cells.红霉素抑制生长中的大肠杆菌细胞中大核糖体亚基的组装。
Curr Microbiol. 1995 May;30(5):273-9. doi: 10.1007/BF00295501.
3
Biosynthesis of 50 s ribosomal subunit in Escherichia coli.大肠杆菌中50 s核糖体亚基的生物合成。
J Mol Biol. 1969 Mar 28;40(3):321-51. doi: 10.1016/0022-2836(69)90158-2.
4
On the nature of antibiotic binding sites in ribosomes.论核糖体中抗生素结合位点的性质
Biochimie. 1987 Aug;69(8):863-9. doi: 10.1016/0300-9084(87)90213-6.
5
Lack of complete cooperativity of ribosome assembly in vitro and its possible relevance to in vivo ribosome assembly and the regulation of ribosomal gene expression.体外核糖体组装缺乏完全协同性及其与体内核糖体组装和核糖体基因表达调控的可能关联。
Biochimie. 1991 Jun;73(6):757-67. doi: 10.1016/0300-9084(91)90055-6.
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