Allen J W, Shuler C F, Mendes R W, Latt S A
Cytogenet Cell Genet. 1977;18(4):231-7. doi: 10.1159/000130765.
A small tablet of 5-bromodeoxyuridine (BrdU), implanted subcutaneously in a mouse, provides sustained release of base analog sufficient to effect substitution of DNA throughout an entire replication period. As illustrated by studies of mouse bone-marrow and spleen cells in the presence or absence of cyclophosphamide, this depot method of BrdU administration greatly simplifies in vivo analysis of sister-chromatid-exchange formation.
将一小片5-溴脱氧尿苷（BrdU）皮下植入小鼠体内，可实现碱基类似物的持续释放，足以在整个复制期内影响DNA的替代。如对存在或不存在环磷酰胺情况下的小鼠骨髓和脾细胞的研究所表明的，这种BrdU给药的储库方法极大地简化了体内姐妹染色单体交换形成的分析。
