Marshall G E, Shehab S A, Spike R C, Todd A J
Laboratory of Human Anatomy, University of Glasgow, UK.
Neuroscience. 1996 May;72(1):255-63. doi: 10.1016/0306-4522(95)00558-7.
In order to determine whether spinothalamic neurons in the lumbar spinal cord of the rat process neurokinin-1 (substance P) receptors, we injected cholera toxin B subunit into the thalamus and carried out dual-labelling immunocytochemistry to search for neurons that were immunoreactive with antibodies to cholera toxin and neurokinin-1 receptor. We examined 356 spinothalamic neurons in transverse sections and found that 35% of these were neurokinin-1 receptor-immunoreactive. Double-labelled cells made up the majority of the spinothalamic population in lamina I and the lateral spinal nucleus, and were also present in laminae III-V and the area around the central canal. On the side contralateral to the injection site, 77% of spinothalamic neurons in lamina I also showed neurokinin-1 receptor immunoreactivity, while 33% of those in laminae III-V and 14% of the ventromedial group possessed the receptor. Several of the double-labelled neurons with cell bodies in laminae III and IV had dendrites which could be followed dorsally into the superficial dorsal horn. These results indicate that substance P released from nociceptive primary afferents into the superficial dorsal horn is likely to act on spinothalamic tract neurons in lamina I, and also on those with cells bodies in laminae III-IV and long dorsal dendrites.
为了确定大鼠腰脊髓中的脊髓丘脑束神经元是否表达神经激肽-1（P物质）受体，我们将霍乱毒素B亚单位注入丘脑，并进行双重标记免疫细胞化学，以寻找对霍乱毒素抗体和神经激肽-1受体呈免疫反应的神经元。我们在横切面上检查了356个脊髓丘脑束神经元，发现其中35%对神经激肽-1受体呈免疫反应。双重标记的细胞在I层和脊髓外侧核中占脊髓丘脑束神经元的大多数，也存在于III-V层和中央管周围区域。在注射部位对侧，I层中77%的脊髓丘脑束神经元也显示出神经激肽-1受体免疫反应性，而III-V层中33%的神经元和腹内侧组中14%的神经元具有该受体。一些胞体位于III层和IV层的双重标记神经元有树突，可追踪到背侧浅层脊髓后角。这些结果表明，伤害性初级传入纤维释放到浅层脊髓后角的P物质可能作用于I层的脊髓丘脑束神经元，也作用于胞体位于III-IV层且有长背侧树突的神经元。
