da Cunha R V, Dias M, Nogueira R M, Chagas N, Miagostovich M P, Schatzmayr H G
Departamento de Clínica Médica, Universidade Federal de Mato Grosso do Sul, Rio de Janeiro, Brazil.
Rev Inst Med Trop Sao Paulo. 1995 Nov-Dec;37(6):517-21. doi: 10.1590/s0036-46651995000600008.
A seroepidemiologic survey was carried out in schoolchildren from public schools of the Niterói municipality, state of Rio de Janeiro, Brazil, after a period of sequential epidemics by dengue virus type 1 and 2 (DEN-1 and DEN-2). 450 blood samples were obtained by fingertip puncture and collected on filter paper discs. The hemagglutination inhibition (HAI) test was carried out using DEN-1 and DEN-2 antigens. HAI titres were demonstrated in 66% (297/450) of the sera and the geometric means of the titres were 1/182 and 1/71 for DEN-1 and DEN-2, respectively. Secondary infections were observed in 61% (181/297) of positive cases. Among these, 75% (135/181) were under fifteen years old. No dengue haemorrhagic fever (DHF) was reported in these children. Asymptomatic or oligosymptomatic infections were detected in 56% of the studied population. The absolute and relative frequencies of positive tests by age group and sex did not evidence statistically significant difference. The number of individuals infected probably produced a immunologic barrier responsible for the non occurrence of dengue epidemic in the latter years.
在巴西里约热内卢州尼泰罗伊市公立学校的学童中开展了一项血清流行病学调查，该调查是在1型和2型登革热病毒（DEN-1和DEN-2）相继流行一段时间之后进行的。通过指尖采血获取450份血样，并收集在滤纸圆盘上。使用DEN-1和DEN-2抗原进行血凝抑制（HAI）试验。66%（297/450）的血清中显示有HAI滴度，DEN-1和DEN-2的滴度几何均值分别为1/182和1/71。在61%（181/297）的阳性病例中观察到二次感染。其中，75%（135/181）为15岁以下儿童。这些儿童中未报告登革出血热（DHF）。在56%的研究人群中检测到无症状或症状轻微的感染。按年龄组和性别划分的阳性检测绝对频率和相对频率均未显示出统计学上的显著差异。感染个体的数量可能形成了一道免疫屏障，导致随后几年未发生登革热疫情。
