Wu T C, He H Z, Tanguay R M, Wu Y, Xu D G, Currie R W, Qu S, Feng J D, Zhang G G
Institute of Occupational Medicine, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1995;15(3):178-83. doi: 10.1007/BF02888231.
In this study, we have examined the effects of exposure to high temperature, carbon monoxide or a combination of both conditions in a model system, the rat and in industrial workers. In the rat liver, HSP70 mRNA and HSP70 synthesis were measured by dot hybridization and western blot. The results showed that after a heat stress HSP70 mRNA and its product, HSP70 increased significantly and there was a synergism in the combined effects of high temperature and carbon monoxide exposure on the induction of HSP70 mRNA and HSP70 synthesis. Heat played a major role in this induction. The presence of antibodies to human HSP27, HSP60, HSP70, HSC73, HSP89 alpha and beta in workers exposed to heat, carbon monoxide was also measured by western blot using purified HSPs as antigens. Plasma free amino acids were measured in the same group of workers. The incidence of antibodies to HSP27 and HSP70 was significantly higher in the workers working in an environment with extreme heat, and high carbon monoxide emission than in a control group. The carbon monoxide exposed group showed the highest incidence of antibodies to HSPs. Although our previous results indicated that workers had an insufficient protein intake, plasma free amino acids tended to increase, especially in methionine and tryptophan two kinds of amino acids which are absent from the main stress protein, HSP70. These results suggest that the major problems that these workers may face are how to facilitate the use of plasma free amino acids and reduce the inhibition of synthesis of normal proteins when they are exposed to occupational harmful factors. These results also add new information on the measurement of HSPs as a potential biomonitor to assess whether organisms are experiencing metabolic stress within their environment.
在本研究中，我们在大鼠这一模型系统以及产业工人中，研究了暴露于高温、一氧化碳或这两种条件的组合所产生的影响。在大鼠肝脏中，通过斑点杂交和蛋白质印迹法测量HSP70 mRNA和HSP70的合成。结果显示，热应激后HSP70 mRNA及其产物HSP70显著增加，并且高温和一氧化碳暴露对HSP70 mRNA诱导和HSP70合成的联合作用存在协同效应。热在这种诱导中起主要作用。还通过使用纯化的热休克蛋白（HSP）作为抗原的蛋白质印迹法，检测了暴露于热、一氧化碳的工人中针对人HSP27、HSP60、HSP70、HSC73、HSP89α和β的抗体的存在情况。在同一组工人中测量了血浆游离氨基酸。在极端高温且一氧化碳排放高的环境中工作的工人，其针对HSP27和HSP70的抗体发生率显著高于对照组。一氧化碳暴露组显示出针对热休克蛋白的抗体发生率最高。尽管我们之前的结果表明工人蛋白质摄入量不足，但血浆游离氨基酸有增加的趋势，尤其是在主要应激蛋白HSP70中不存在的蛋氨酸和色氨酸这两种氨基酸。这些结果表明，这些工人可能面临的主要问题是，当他们暴露于职业有害因素时，如何促进血浆游离氨基酸的利用并减少对正常蛋白质合成的抑制。这些结果还为将热休克蛋白作为一种潜在生物监测指标以评估生物体在其环境中是否经历代谢应激的测量增加了新信息。
