Conway E J, Hudnall S D, Lazarides A, Bahler A, Fraire A E, Cagle P T
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
Mod Pathol. 1996 May;9(5):491-5.
Although most lung carcinomas and mesotheliomas are associated with well-known risk factors, these cancers develop in only a minority of persons exposed to known risk factors. On the other hand, these cancers develop in some patients without exposure to known risk factors. This indicates that other environmental factors play a role in the carcinogenesis of these tumors. Oncogenic viruses such as Epstein-Barr virus (EBC) are well-established agents in the development of certain cancers. EBV genomes have been detected by in situ hybridization in gastric adenocarcinomas and in nasopharyngeal carcinomas. To determine whether EBV infection is associated with pulmonary adenocarcinoma or mesothelioma, we performed EBV-encoded RNA-1 in situ hybridization on 80 pulmonary adenocarcinoma and 50 mesothelioma resection specimens. Sections were cut from paraffin-embedded tissue and EBV-encoded RNA-1 in situ hybridization was performed using an antisense oligoprobe. Sections were reviewed for the presence of EBV-encoded RNA-1 in tumor cells. All 80 adenocarcinomas and 50 mesotheliomas were negative for EBV-encoded RNA-1 by in situ hybridization. In conclusion, no evidence for an etiologic role for EBV in the development of pulmonary adenocarcinoma or pleural mesothelioma was found in this study.
尽管大多数肺癌和间皮瘤都与众所周知的风险因素相关，但这些癌症仅在少数接触已知风险因素的人群中发生。另一方面，这些癌症也会在一些未接触已知风险因素的患者中出现。这表明其他环境因素在这些肿瘤的致癌过程中起作用。致癌病毒，如爱泼斯坦-巴尔病毒（EBC），是某些癌症发生发展中已明确的因素。在胃腺癌和鼻咽癌中，已通过原位杂交检测到EBV基因组。为了确定EBV感染是否与肺腺癌或间皮瘤相关，我们对80例肺腺癌和50例间皮瘤切除标本进行了EBV编码RNA-1原位杂交。从石蜡包埋组织中切片，使用反义寡核苷酸探针进行EBV编码RNA-1原位杂交。检查切片以确定肿瘤细胞中是否存在EBV编码RNA-1。通过原位杂交，所有80例腺癌和50例间皮瘤的EBV编码RNA-1均为阴性。总之，本研究未发现EBV在肺腺癌或胸膜间皮瘤发生中起病因学作用的证据。
