细胞培养、小鼠接种和聚合酶链反应检测刚地弓形虫的比较：储存条件对灵敏度的影响
Comparison of cell culture, mouse inoculation, and PCR for detection of Toxoplasma gondii: effects of storage conditions on sensitivity.
作者信息
James G S, Sintchenko V G, Dickeson D J, Gilbert G L
机构信息
Department of Clinical Microbiology, Westmead Hospital, New South Wales, Australia.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1572-5. doi: 10.1128/jcm.34.6.1572-1575.1996.
Abstract
The sensitivity of detection of a wild-type strain of Toxoplasma gondii by cell culture, mouse inoculation, and PCR was determined following sample storage under conditions to which clinical specimens may be subjected during transport to the testing laboratory. Sample storage at -20 degrees C significantly decreased the sensitivity of mouse inoculation. The sensitivity of cell culture decreased with sample storage at 4 and -20 degrees C. The sensitivity of PCR was reduced by storage at 4 degrees C for 48 h, freezing, and heating. These findings have implications for the selection of appropriate methods for the direct detection of T. gondii organisms in suboptimally transported clinical samples.
摘要
在将临床标本运送至检测实验室过程中可能遇到的条件下储存样本后，测定了通过细胞培养、小鼠接种和聚合酶链反应（PCR）检测野生型弓形虫菌株的敏感性。在-20℃储存样本显著降低了小鼠接种的敏感性。细胞培养的敏感性随着样本在4℃和-20℃储存而降低。PCR的敏感性因在4℃储存48小时、冷冻和加热而降低。这些发现对于选择合适的方法直接检测运输条件欠佳的临床样本中的弓形虫生物体具有重要意义。
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