金黄色葡萄球菌M株表面多糖的结构分析
Structural analysis of the surface polysaccharide of Staphylococcus aureus M.
作者信息
Liau D F, Hash J H
出版信息
J Bacteriol. 1977 Jul;131(1):194-200. doi: 10.1128/jb.131.1.194-200.1977.
Abstract
The chemical structure of the surface polysaccharide from Staphylococcus aureus M was investigated by a combination of methanolytic, hydrolytic, and chromatographic techniques. The repeating unit that was most consistent with the data was a hexasaccharide composed of N-acetyl-D-aminogalacturonic acid, N-acetyl-D-fucosamine, and taurine in molar ratios of 4:2:1. A disaccharide was isolated and characterized, by combined gas-liquid chromatography-mass spectrometry, as N-acetyl-D-aminogalacturonyl-(1 leads to 3)-N-acetyl-D-fucosamine. Taurine is linked to a carboxyl group of N-acetyl-D-aminogalacturonic acid via an amide bond.
摘要
通过甲醇分解、水解和色谱技术相结合的方法，对金黄色葡萄球菌M表面多糖的化学结构进行了研究。与数据最相符的重复单元是一种六糖，由N-乙酰-D-氨基半乳糖醛酸、N-乙酰-D-岩藻糖胺和牛磺酸按4:2:1的摩尔比组成。通过气液色谱-质谱联用技术分离并鉴定出一种二糖，为N-乙酰-D-氨基半乳糖醛酸基-(1→3)-N-乙酰-D-岩藻糖胺。牛磺酸通过酰胺键与N-乙酰-D-氨基半乳糖醛酸的羧基相连。
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