Błaszczyk B, Inglot A D, Kowalczyk-Bronisz S H, Szymaniec S, Młochowski J
Laboratory of Immunopharmacology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Arch Immunol Ther Exp (Warsz). 1995;43(5-6):305-11.
We have investigated the immunotropic effects of 23 seleno-organic compounds (8 benzisoselenazolones, 3 benzisoselenazolone oxides and 12 organic diselenides). All of the compounds increased the rosette formation of sheep red blood cells (SRBC) with spleen cells obtained from thymectomized C53BL/6 mice and incubated in vitro in the presence of imuran. Furthermore, 16 of the compounds were also assayed in vitro in the hydrocortisone test performed with C57BL/6 mouse thymocytes. It was found that all of them significantly protected the cells against hydrocortisone induced cytotoxicity. Also in the Jerne's assay, performed in 129Ao/Boy mice pretreated in vivo with 3 selected compounds 5 days before immunization with SRBC, the stimulation of plaque forming cells (PFC) was observed. Only one compound (AE22, an analog of piroxicam) was found to be inhibitory in this assay. In contrast, in the graft versus host (GvH) assay performed in hybrid mice the donor lymphoid cells obtained from C57BL/6 mice pretreated with 9 selected seleno-organic compounds, suppressed the GvH reaction in the recipient hybrid mice. Thus, in all of the immunotropic assays except the GvH reaction in adult mice, the seleno-organic compounds were found to have immunostimulating activities.
我们研究了23种有机硒化合物（8种苯并异硒唑酮、3种苯并异硒唑酮氧化物和12种有机二硒化物）的免疫调节作用。所有这些化合物都能增强经胸腺切除的C53BL/6小鼠脾脏细胞与绵羊红细胞（SRBC）的玫瑰花结形成，这些细胞在体外于硫唑嘌呤存在的情况下进行培养。此外，其中16种化合物还在以C57BL/6小鼠胸腺细胞进行的氢化可的松试验中进行了体外检测。结果发现，所有这些化合物都能显著保护细胞免受氢化可的松诱导的细胞毒性。同样在Jerne试验中，在用3种选定化合物于体内预处理129Ao/Boy小鼠5天后，再用SRBC进行免疫，观察到了对空斑形成细胞（PFC）的刺激。仅有一种化合物（AE22，吡罗昔康类似物）在此试验中表现出抑制作用。相反，在杂交小鼠中进行的移植物抗宿主（GvH）试验中，从用9种选定有机硒化合物预处理的C57BL/6小鼠获得的供体淋巴细胞，抑制了受体杂交小鼠中的GvH反应。因此，在除成年小鼠GvH反应之外的所有免疫调节试验中，发现有机硒化合物都具有免疫刺激活性。
