两种用于巨细胞病毒抗原血症检测的白细胞提取方法的比较。
Comparison of two leukocyte extraction methods for cytomegalovirus antigenemia assay.
作者信息
García A, Niubò J, Benítez M A, Viqueira M, Pérez J L
机构信息
Servicio de Microbiología, Hospital Prínceps d'Espanya, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.
出版信息
J Clin Microbiol. 1996 Jan;34(1):182-4. doi: 10.1128/jcm.34.1.182-184.1996.
Abstract
We carried out a prospective, parallel, and blind study on 113 blood samples from immunocompromised patients in order to compare two leukocyte extraction methods (6% dextran sedimentation and Polymorphprep separation) for cytomegalovirus (CMV) antigenemia assay. CMV was detected in 38 samples by antigenemia assay (34 by dextran sedimentation and 35 by Polymorphprep separation). No differences either in the number of positive specimens (P = 1) or in the mean CMV-positive cell counts (P = 0.41) were observed between the two leukocyte extraction methods. In conclusion, the two methods performed equally well for this assay.
摘要
我们对113份来自免疫功能低下患者的血样进行了一项前瞻性、平行且盲法的研究，以比较两种白细胞提取方法（6%葡聚糖沉降法和聚蔗糖-泛影葡胺分离法）用于巨细胞病毒（CMV）抗原血症检测的效果。通过抗原血症检测在38份样本中检测到了CMV（葡聚糖沉降法检测出34份，聚蔗糖-泛影葡胺分离法检测出35份）。在两种白细胞提取方法之间，无论是阳性标本数量（P = 1）还是平均CMV阳性细胞计数（P = 0.41）均未观察到差异。总之，这两种方法在该检测中表现相当。
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