Wu C H, Luo S F, Wong D W
Department of Medical Research, Taichung Veterans General Hospital, Taipei, Taiwan, Republic of China.
Allergy. 1997 Apr;52(4):411-6. doi: 10.1111/j.1398-9995.1997.tb01020.x.
Serum from atopics hypersensitive to the American cockroach were examined for their specific IgE to American and German cockroaches by the fluoroallergosorbent test (FAST). Of 44 sera tested, 86.4% (38/44) contained IgE to American and German cockroaches, and 13.6% (6/44) were found to be positive for American cockroach alone by FAST. Nine individual sera containing IgE antibodies to both cockroaches were used to analyze the cross-reacting allergens in the crude American and German cockroach extracts by FAST inhibition and immunoblotting FAST-inhibition studies showed various degrees but similar inhibition of binding of human IgE to solid-phase American cockroach extract. Proteins from both cockroach extracts were separated on SDS-PAGE followed by immunoblotting, and the results showed considerable heterogeneity in the IgE-binding patterns with each of the cockroach extracts for the same nine individual sera. Components with apparent molecular weights of 60, 52, 49, 38, and 12 kDa from both the American and German cockroaches were able to bind IgE antibody. These results suggest the presence of cross-reactive allergens in the American and the German cockroaches.
通过荧光变应原吸附试验（FAST）检测对美洲蟑螂过敏的特应性个体血清中针对美洲蟑螂和德国蟑螂的特异性IgE。在检测的44份血清中，86.4%（38/44）含有针对美洲蟑螂和德国蟑螂的IgE，通过FAST发现13.6%（6/44）仅对美洲蟑螂呈阳性。使用9份含有针对两种蟑螂的IgE抗体的个体血清，通过FAST抑制和免疫印迹分析美洲蟑螂和德国蟑螂粗提物中的交叉反应性变应原。FAST抑制研究显示人IgE与固相美洲蟑螂提取物结合的抑制程度各异但相似。两种蟑螂提取物中的蛋白质经SDS-PAGE分离后进行免疫印迹，结果显示，对于相同的9份个体血清，每种蟑螂提取物的IgE结合模式存在相当大的异质性。来自美洲蟑螂和德国蟑螂的表观分子量为60、52、49、38和12 kDa的成分能够结合IgE抗体。这些结果表明美洲蟑螂和德国蟑螂中存在交叉反应性变应原。
