Wahle E, Keller W
Institut für Biochemie, Universität Giessen, Germany.
Trends Biochem Sci. 1996 Jul;21(7):247-50.
During the synthesis of mRNA in the nucleus, 3'-ends are generated by endonucleolytic cleavage followed by polyadenylation. The machinery responsible for this simple reaction is surprisingly complex. In vitro reconstitution of 3'-end processing has demonstrated the importance of cooperative interactions in RNA recognition and catalysis. However, the inventory of processing factors is still incomplete and important mechanistic questions have not yet been answered.
在细胞核中合成信使核糖核酸（mRNA）的过程中，3' 端是通过核酸内切酶切割后再进行聚腺苷酸化而产生的。负责这一简单反应的机制惊人地复杂。体外重建 3' 端加工过程已证明了协同相互作用在 RNA 识别和催化中的重要性。然而，加工因子的清单仍不完整，重要的机制问题尚未得到解答。
