一个新的CcpA结合位点在……的碳分解代谢中起双向作用。
A new CcpA binding site plays a bidirectional role in carbon catabolism in .
作者信息
Xiao Fengxu, Li Youran, Zhang Yupeng, Wang Hanrong, Zhang Liang, Ding Zhongyang, Gu Zhenghua, Xu Sha, Shi Guiyang
机构信息
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, People's Republic of China.
出版信息
iScience. 2021 Apr 7;24(5):102400. doi: 10.1016/j.isci.2021.102400. eCollection 2021 May 21.
is widely used to produce various valuable products, such as food enzymes, industrial chemicals, and biocides. The carbon catabolite regulation process in the utilization of raw materials is crucial to maximizing the efficiency of this microbial cell factory. The current understanding of the molecular mechanism of this regulation is based on limited motif patterns in protein-DNA recognition, where the typical catabolite-responsive element (CRE) motif is "TGWNANCGNTNWCA". Here, CRE is identified and characterized as a new CRE. It consists of two palindrome arms of 6 nucleotides (AGCTTT/AAAGCT) and an intermediate spacer. CRE is involved in bidirectional regulation in a glucose stress environment. When AGCTTT appears in the 5' end, the regulatory element exhibits a carbon catabolite activation effect, while AAAGCT in the 5' end corresponds to carbon catabolite repression. Further investigation indicated a wide occurrence of CRE in the genome of .
广泛用于生产各种有价值的产品，如食品酶、工业化学品和生物杀灭剂。在原材料利用过程中的碳分解代谢物调控过程对于最大化这个微生物细胞工厂的效率至关重要。目前对这种调控分子机制的理解基于蛋白质-DNA识别中有限的基序模式，其中典型的分解代谢物反应元件（CRE）基序是“TGWNANCGNTNWCA”。在此，CRE被鉴定并表征为一种新的CRE。它由两个6个核苷酸的回文臂（AGCTTT/AAAGCT）和一个中间间隔区组成。CRE在葡萄糖应激环境中参与双向调控。当AGCTTT出现在5'端时，调控元件表现出碳分解代谢物激活效应，而5'端的AAAGCT则对应碳分解代谢物阻遏。进一步研究表明CRE在……的基因组中广泛存在。
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