Jin L Z, Ho Y W, Ali M A, Abdullah N, Jalaludin S
Department of Animal Science, Universiti Pertanian Malaysia, Serdang, Selangor, Malaysia.
J Appl Bacteriol. 1996 Aug;81(2):201-6. doi: 10.1111/j.1365-2672.1996.tb04501.x.
Single strains of Lactobacillus acidophilus and Lact. fermentum, isolated from chicken intestine, were used to study in vitro interactions with Salmonella enteritidis, Salm. pullorum or Salm. typhimurium in an ileal epithelial cell (IEC) radioactive assay. Exclusion, competition and displacement phenomena were investigated by respectively incubating (a) lactobacilli and IEC together, prior to addition of salmonellae, (b) lactobacilli, IEC and salmonellae together, and (c) salmonellae and IEC, followed by the lactobacilli. Lactobacilli were selected for study because of their strong ability to adhere to IEC and poor aggregation with salmonellae. The results demonstrated that Lact. acidophilus significantly reduced (P < 0.05) the attachment of Salm. pullorum to IEC in the tests for exclusion and competition, but not in the displacement tests. Lactobacillus fermentum was found to have some ability to reduce the attachment of Salm. typhimurium to IEC under the conditions of exclusion (P < 0.08), competition (P < 0.09), but not displacement. However, both Lact. acidophilus and Lact. fermentum were unable to reduce the adherence of Salm. enteritidis to IEC under any of the conditions.
从鸡肠道分离出的嗜酸乳杆菌和发酵乳杆菌单菌株，用于在回肠上皮细胞（IEC）放射性测定中研究与肠炎沙门氏菌、鸡白痢沙门氏菌或鼠伤寒沙门氏菌的体外相互作用。通过分别进行以下孵育来研究排斥、竞争和取代现象：（a）在添加沙门氏菌之前，将乳杆菌和IEC一起孵育；（b）将乳杆菌、IEC和沙门氏菌一起孵育；（c）将沙门氏菌和IEC孵育，然后加入乳杆菌。选择乳杆菌进行研究是因为它们具有很强的粘附IEC的能力，并且与沙门氏菌的聚集性较差。结果表明，在排斥和竞争试验中，嗜酸乳杆菌显著降低（P < 0.05）鸡白痢沙门氏菌对IEC的附着，但在取代试验中没有。在排斥（P < 0.08）、竞争（P < 0.09）条件下，发现发酵乳杆菌有一定能力降低鼠伤寒沙门氏菌对IEC的附着，但在取代试验中没有。然而，在任何条件下，嗜酸乳杆菌和发酵乳杆菌都不能降低肠炎沙门氏菌对IEC的粘附。
