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来自落羽杉(Taxodium distichum)的产紫杉醇内生真菌。

Endophytic taxol-producing fungi from bald cypress, Taxodium distichum.

作者信息

Li J Y, Strobel G, Sidhu R, Hess W M, Ford E J

机构信息

Department of Plant Pathology, Montana State University, Bozeman 59717, USA.

出版信息

Microbiology (Reading). 1996 Aug;142 ( Pt 8):2223-6. doi: 10.1099/13500872-142-8-2223.

DOI:10.1099/13500872-142-8-2223
PMID:8760934
Abstract

Pestalotiopsis microspora occurs as a range of strains in bald cypress, Taxodium distichum. The organisms live as endophytes in the bark, phloem and xylem, and isolates show differences in cultural and microscopic characteristics on common laboratory media. Many of these fungi make taxol as determined by the reactivity of partially purified culture extracts with specific monoclonal antibodies against taxol. In the case of one strain of P. microspora (CP-4), taxol was isolated from culture medium and was shown to be identical to authentic taxol by chromatographic and spectroscopic means.

摘要

小孢拟盘多毛孢在落羽杉(Taxodium distichum)中以一系列菌株的形式存在。这些生物体作为内生菌生活在树皮、韧皮部和木质部中,并且在普通实验室培养基上,分离株在培养和微观特征上表现出差异。通过部分纯化的培养提取物与抗紫杉醇的特异性单克隆抗体的反应性确定,许多这些真菌能产生紫杉醇。就小孢拟盘多毛孢的一个菌株(CP - 4)而言,从培养基中分离出了紫杉醇,并通过色谱和光谱方法证明其与正品紫杉醇相同。

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