Bale J F, Petheram S J, Souza I E, Murph J R
Department of Pediatrics, University of Iowa College of Medicine, Iowa City, USA.
J Pediatr. 1996 Mar;128(3):347-52. doi: 10.1016/s0022-3476(96)70279-2.
To determine the frequency of reinfection with new cytomegalovirus (CMV) strains in children in group child-care environments.
Ninety-two CMV strains isolated serially from children attending child care centers were analyzed. Strains were obtained from 1986 to 1994, from 37 children attending one of six centers in the area of Cedar Rapids and Iowa City, Iowa. The CMV isolates were analyzed by a polymerase chain reaction-based algorithm using primers for the a-sequence, glycoprotein B, and major immediate early (MIE) genes of human CMV. The a-sequence polymerase chain reaction products were compared on the basis of size, and products derived from glycoprotein B and MIE genes were compared according to restriction fragment length polymorphisms.
Children were between 8 months and 5 years 7 months of age at the time of CMV isolation. The number of isolates ranged from 2 to 6 per child, and the intervals between the first and last CMV isolation ranged from 11 weeks to more than 3 years. At least 7 (19%) of the 37 children had evidence of infection with more than one CMV strain. In six of these children, reinfection with distinct strains was confirmed by analysis of the MIE gene products of sequential CMV strains.
Children who attend child care centers, like adults who are immunosuppressed or have multiple sexual partners, are at risk of being reinfected with distinct CMV strains.
确定在集体儿童保育环境中儿童感染新型巨细胞病毒（CMV）毒株的频率。
对从参加儿童保育中心的儿童中连续分离出的92株CMV毒株进行分析。毒株于1986年至1994年从爱荷华州锡达拉皮兹市和爱荷华市地区六个中心之一的37名儿童中获得。使用针对人CMV的α序列、糖蛋白B和主要立即早期（MIE）基因的引物，通过基于聚合酶链反应的算法对CMV分离株进行分析。根据大小比较α序列聚合酶链反应产物，并根据限制性片段长度多态性比较糖蛋白B和MIE基因衍生的产物。
CMV分离时儿童年龄在8个月至5岁7个月之间。每个儿童的分离株数量为2至6株，首次和最后一次CMV分离之间的间隔为11周至3年以上。37名儿童中至少有7名（19%）有感染不止一种CMV毒株的证据。在其中6名儿童中，通过对连续CMV毒株的MIE基因产物进行分析，证实感染了不同毒株。
参加儿童保育中心的儿童，如同免疫抑制的成年人或有多个性伴侣的成年人一样，有再次感染不同CMV毒株的风险。
