评估Vitek GPS-SA卡用于检测对苯唑西林敏感性处于临界状态且缺乏mec的葡萄球菌。
Evaluation of Vitek GPS-SA card for testing of oxacillin against borderline-susceptible staphylococci that lack mec.
作者信息
Knapp C C, Ludwig M D, Washington J A, Chambers H F
机构信息
Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195-5140, USA.
出版信息
J Clin Microbiol. 1996 Jul;34(7):1603-5. doi: 10.1128/JCM.34.7.1603-1605.1996.
Abstract
Fifty-one Staphylococcus aureus strains lacking mec for which oxacillin MICs were 1 to 8 micrograms/ml were tested against oxacillin and the combination of oxacillin and clavulanic acid with the Vitek GPS-SA card, the reference broth microdilution method, and the oxacillin agar screen plate. Of the 51 strains, 44 (86%) did not grow on the oxacillin agar screen plate, broth microdilution MICs were 1 to 2 micrograms/ml, and GPS-SA card MICs were < or = 2 micrograms/ml, with the exception of 3 strains that failed to grow in the card on repeated attempts. Another seven strains did grow on the oxacillin agar screen plate. For four of the latter group of strains, oxacillin broth microdilution MICs were > 4 micrograms/ml and GPS-SA card MICs were > or = 4 micrograms/ml; for the other 3 strains, corresponding MICs were 4 and < or = 2 micrograms/ml, respectively. The GPS-SA card classified 86% of strains as oxacillin susceptible.
摘要
对51株缺乏mec基因且苯唑西林最低抑菌浓度（MIC）为1至8微克/毫升的金黄色葡萄球菌菌株，采用Vitek GPS-SA卡、参考肉汤微量稀释法和苯唑西林琼脂筛选平板，检测它们对苯唑西林以及苯唑西林与克拉维酸组合的敏感性。在这51株菌株中，44株（86%）在苯唑西林琼脂筛选平板上不生长，肉汤微量稀释法测得的MIC为1至2微克/毫升，GPS-SA卡测得的MIC≤2微克/毫升，但有3株经多次尝试在卡中未能生长。另外7株在苯唑西林琼脂筛选平板上生长。对于后一组中的4株菌株，苯唑西林肉汤微量稀释法测得的MIC＞4微克/毫升，GPS-SA卡测得的MIC≥4微克/毫升；对于另外3株菌株，相应的MIC分别为4微克/毫升和≤2微克/毫升。GPS-SA卡将86%的菌株分类为对苯唑西林敏感。
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