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Comparison of ATB staph, rapid ATB staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA.用于检测携带mecA的葡萄球菌中苯唑西林异质性耐药的ATB葡萄球菌法、快速ATB葡萄球菌法、Vitek法和E-test法的比较
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The role of beta-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins.β-内酰胺酶在葡萄球菌对耐青霉素酶青霉素和头孢菌素耐药性中的作用。
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New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity.金黄色葡萄球菌对甲氧西林耐药的新机制：缺乏PBP 2a基因且含有具有修饰青霉素结合能力的正常青霉素结合蛋白的临床分离株。
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评估Vitek GPS-SA卡用于检测对苯唑西林敏感性处于临界状态且缺乏mec的葡萄球菌。

Evaluation of Vitek GPS-SA card for testing of oxacillin against borderline-susceptible staphylococci that lack mec.

作者信息

Knapp C C, Ludwig M D, Washington J A, Chambers H F

机构信息

Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195-5140, USA.

出版信息

J Clin Microbiol. 1996 Jul;34(7):1603-5. doi: 10.1128/JCM.34.7.1603-1605.1996.

DOI:10.1128/JCM.34.7.1603-1605.1996
PMID:8784553
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229078/
Abstract

Fifty-one Staphylococcus aureus strains lacking mec for which oxacillin MICs were 1 to 8 micrograms/ml were tested against oxacillin and the combination of oxacillin and clavulanic acid with the Vitek GPS-SA card, the reference broth microdilution method, and the oxacillin agar screen plate. Of the 51 strains, 44 (86%) did not grow on the oxacillin agar screen plate, broth microdilution MICs were 1 to 2 micrograms/ml, and GPS-SA card MICs were < or = 2 micrograms/ml, with the exception of 3 strains that failed to grow in the card on repeated attempts. Another seven strains did grow on the oxacillin agar screen plate. For four of the latter group of strains, oxacillin broth microdilution MICs were > 4 micrograms/ml and GPS-SA card MICs were > or = 4 micrograms/ml; for the other 3 strains, corresponding MICs were 4 and < or = 2 micrograms/ml, respectively. The GPS-SA card classified 86% of strains as oxacillin susceptible.

摘要

对51株缺乏mec基因且苯唑西林最低抑菌浓度（MIC）为1至8微克/毫升的金黄色葡萄球菌菌株，采用Vitek GPS-SA卡、参考肉汤微量稀释法和苯唑西林琼脂筛选平板，检测它们对苯唑西林以及苯唑西林与克拉维酸组合的敏感性。在这51株菌株中，44株（86%）在苯唑西林琼脂筛选平板上不生长，肉汤微量稀释法测得的MIC为1至2微克/毫升，GPS-SA卡测得的MIC≤2微克/毫升，但有3株经多次尝试在卡中未能生长。另外7株在苯唑西林琼脂筛选平板上生长。对于后一组中的4株菌株，苯唑西林肉汤微量稀释法测得的MIC＞4微克/毫升，GPS-SA卡测得的MIC≥4微克/毫升；对于另外3株菌株，相应的MIC分别为4微克/毫升和≤2微克/毫升。GPS-SA卡将86%的菌株分类为对苯唑西林敏感。