Jahn H U, Ullrich R, Schneider T, Liehr R M, Schieferdecker H L, Holst H, Zeitz M
Department of Medicine, Benjamin Franklin Clinic, Free University of Berlin, Germany.
Digestion. 1996;57(2):95-104. doi: 10.1159/000201320.
Saccharomyces boulardii (S.b.) is used for the prevention and treatment of diarrhea of different etiologies. We prospectively investigated the effects of S.b. on lymphocytes and duodenal mucosa. Before and after oral administration of S.b. for 3 weeks, circulating and intestinal lymphocytes were isolated and characterized by flow cytometry. Trophic effects on duodenal mucosa were investigated by morphometry and determination of brush border enzyme activity. Results were compared intraindividually before and after S.b. In intestinal lymphocytes no phenotypic changes were observed. CD4+ cells of the peripheral blood had a significantly increased expression of CD25 (p < 0.02). None of twelve volunteers had an increase in villous surface area (n.s.). Immunoglobulin A content in small intestine secretion was unaltered. An increase in brush border enzyme activity of lactase, alpha-glucosidase, and alkaline phosphatase was observed (p < 0.01). Our findings indicate that S.b. has a positive effect on the maturation of enterocytes and only a minor influence on lymphocytes.
布拉氏酵母菌（S.b.）用于预防和治疗不同病因引起的腹泻。我们前瞻性地研究了S.b.对淋巴细胞和十二指肠黏膜的影响。口服S.b. 3周前后，分离循环和肠道淋巴细胞，并通过流式细胞术进行表征。通过形态计量学和刷状缘酶活性测定研究对十二指肠黏膜的营养作用。结果在个体内比较S.b.前后的情况。在肠道淋巴细胞中未观察到表型变化。外周血CD4 +细胞的CD25表达显著增加（p < 0.02）。十二名志愿者中无一例绒毛表面积增加（无显著性差异）。小肠分泌物中的免疫球蛋白A含量未改变。观察到乳糖酶、α-葡萄糖苷酶和碱性磷酸酶的刷状缘酶活性增加（p < 0.01）。我们的研究结果表明，S.b.对肠细胞成熟有积极作用，对淋巴细胞影响较小。
