Bikandi J, Millán R S, Moragues M D, Cebas G, Clarke M, Coleman D C, Sullivan D J, Quindós G, Pontón J
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, E-48080 Bilbao, Vizcaya, Spain.
J Clin Microbiol. 1998 Sep;36(9):2428-33. doi: 10.1128/JCM.36.9.2428-2433.1998.
There is a clear need for the development of a rapid and reliable test for the identification of Candida dubliniensis and for the discrimination of this species from Candida albicans. In the present study we have investigated the potential use of C. dubliniensis-specific antigens as a basis for its identification. We produced an anti-C. dubliniensis serum which, after adsorption with C. albicans blastospores, was found to differentially label C. dubliniensis isolates in an indirect immunofluorescence test. In this test, the antiserum reacted with blastospores and germ tubes of C. dubliniensis and with blastospores of Candida krusei and Rhodotorula rubra but did not react with blastospores of several other Candida species including C. albicans. The antiserum also reacted with C. albicans germ tubes. The anti-C. dubliniensis adsorbed serum reacted with specific components of 25, 28, 37, 40, 52, and 62 kDa in the C. dubliniensis extract and with a variety of antigens from other yeast species. The antigens from non-C. dubliniensis yeasts showing reactivity with the anti-C. dubliniensis adsorbed serum are mostly expressed within the cell walls of these yeast species, and this reactivity does not interfere with the use of the anti-C. dubliniensis adsorbed serum in an indirect immunofluorescence test for the rapid identification of C. dubliniensis.
显然需要开发一种快速可靠的检测方法来鉴定都柏林念珠菌，并将该菌种与白色念珠菌区分开来。在本研究中，我们调查了都柏林念珠菌特异性抗原作为其鉴定依据的潜在用途。我们制备了一种抗都柏林念珠菌血清，在用白色念珠菌芽生孢子吸附后，发现其在间接免疫荧光试验中能特异性标记都柏林念珠菌分离株。在该试验中，抗血清与都柏林念珠菌的芽生孢子和芽管以及克鲁斯念珠菌和深红酵母的芽生孢子发生反应，但不与包括白色念珠菌在内的其他几种念珠菌的芽生孢子发生反应。该抗血清也与白色念珠菌的芽管发生反应。抗都柏林念珠菌吸附血清与都柏林念珠菌提取物中25、28、37、40、52和62 kDa的特定成分以及其他酵母菌种的多种抗原发生反应。与抗都柏林念珠菌吸附血清发生反应的非都柏林念珠菌酵母的抗原大多在这些酵母菌种的细胞壁内表达，并且这种反应性不影响抗都柏林念珠菌吸附血清在间接免疫荧光试验中用于快速鉴定都柏林念珠菌。
