Heller R, Jaulhac B, Charles P, De Briel D, Vincent V, Bohner C, Piémont Y, Monteil H
Institut de Bactériologie, Faculté de Médecine, Strasbourg, France.
Eur J Clin Microbiol Infect Dis. 1996 Feb;15(2):172-5. doi: 10.1007/BF01591494.
A new, well-documented case of Mycobacterium shimoidei infection is reported. This bacterium was isolated from a patient with preexisting pulmonary lesions who developed a tuberculosis-like cavity in the lung. Isolation of fewer than ten strains, not epidemiologically related, has been reported worldwide; all originated from human pulmonary samples. Identification of the strain described here was first accomplished by 16S rDNA direct sequencing after in vitro amplification. This method allows rapid identification of unusual bacteria. The identification was confirmed by specific phenotypical tests and by chromatographic methods.
本文报告了一例新的、有充分记录的嗜沫分枝杆菌感染病例。该细菌从一名已有肺部病变并在肺部出现类似结核空洞的患者中分离得到。全球范围内报道的分离出少于十株且无流行病学关联的菌株,均源自人类肺部样本。此处描述的菌株首先通过体外扩增后的16S rDNA直接测序进行鉴定。该方法可快速鉴定异常细菌。通过特定的表型试验和色谱方法对鉴定结果进行了确认。