Rao G G, Ghanekar K, Ojo F
Lewisham Hospital, NHS Trust, London, UK.
Eur J Clin Microbiol Infect Dis. 1996 Feb;15(2):175-7. doi: 10.1007/BF01591495.
Infections caused by vancomycin-resistant enterococci (VRE) are becoming increasingly prevalent throughout the world. Control measures include detection and isolation of carriers of VRE. A selective medium to detect faecal carriage of VRE is described. The medium has a high productivity ratio (90.4%) for VRE with VanA resistance phenotype, a moderate productivity ratio (79.2%) for VRE with VanB resistance phenotype, and a relatively low productivity ratio (65.5%) for VRE with VanC resistance phenotype. There was no breakthrough of vancomycin-susceptible enterococci. The medium selected the growth of all three types of VRE, which were used to spike faecal specimens. In a limited clinical trial, six faecal specimens of carriers and contacts were screened using the selective medium. Vancomycin-resistant enterococci (Enterococcus faecalis, VanA phenotype) were detected in four of the specimens. In all four specimens the growth of VRE was nearly pure and easily identifiable.
耐万古霉素肠球菌（VRE）引起的感染在全球范围内日益普遍。控制措施包括检测和隔离VRE携带者。本文描述了一种用于检测粪便中VRE携带情况的选择性培养基。该培养基对具有VanA耐药表型的VRE具有较高的检出率（90.4%），对具有VanB耐药表型的VRE具有中等检出率（79.2%），而对具有VanC耐药表型的VRE检出率相对较低（65.5%）。对万古霉素敏感的肠球菌未出现漏检情况。该培养基能促进用于接种粪便标本的所有三种类型VRE的生长。在一项有限的临床试验中，使用该选择性培养基对6份携带者和接触者的粪便标本进行了筛查。在4份标本中检测到了耐万古霉素肠球菌（粪肠球菌，VanA表型）。在所有4份标本中，VRE的生长几乎呈纯种且易于识别。
